Involvement of indole-3-acetic acid produced by the growth-promoting bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris
Involvement of indole-3-acetic acid produced by the growth-promoting bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris
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DOI：
10.1111/j.1529-8817.2008.00533.x
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发表时间：
2008-08-01
影响因子：
2.9
通讯作者：
Bashan, Yoav
中科院分区：
文献类型：
--
作者：
de-Bashan, Luz E.;Antoun, Hani;Bashan, Yoav
微藻促生菌巴西氮螺旋菌和脂孢微藻产生的吲哚-3-乙酸（IAA）在促进普通小球藻生长中的作用。进行了研究。将4株偶氮螺旋菌野生型菌株及其iaa缺陷突变体与C. vulgaris共固定在海藻酸珠中。培养物在添加色氨酸的合成培养基中生长。监测了微藻的生长促进和偶氮螺旋藻产生外源IAA的情况。所有野生型氮螺旋藻都产生了显著但不同数量的IAA，而它们的突变型产生的IAA明显较少。结果表明，四种氮螺旋藻野生型菌株对小球藻培养物的生长有显著的促进作用，而四种缺乏iaa的突变体对小球藻生长的促进作用非常低或没有，与单独固定的小球藻相比。在一项互补实验中，将IAA减弱突变体巴西芽孢杆菌SpM7918与其亲本野生型菌株巴西芽孢杆菌Sp6产生的IAA进行补充，在微藻突变体培养中恢复了生长促进作用。
Involvement of indole-3-acetic acid (IAA), produced by the microalgae-growth-promoting bacteria Azospirillum brasilens and A. lipoferum, in promoting growth of the microalga Chlorella vulgaris Beij. was studied. Four wildtype strains of Azospirillum and their IAA-deficient mutants were co-immobilized with C. vulgaris in alginate beads. Cultures were grown in synthetic growth medium supplemented with tryptophan. Growth promotion of microalgae and production of exogenous IAA by Azospirillum spp. were monitored. All wildtype Azospirillum spp. produced significant but varying amounts of IAA, while their mutant forms produced significantly less. The results demonstrated a significant growth promotion in Chlorella cultures when immobilized with the four wildtype strains of Azospirillum, while very low or no enhanced growth was induced by the four IAA-deficient mutants, compared to when C. vulgaris is immobilized alone. A complementation experiment, where an IAA-attenuated mutant (A. brasilense SpM7918) was supplemented with IAA produced by its parental wildtype strain (A. brasilense Sp6), restored growth promotion in the microalgae-mutant culture.