Continuous flow nanoparticle concentration using alternating current-electroosmotic flow
Continuous flow nanoparticle concentration using alternating current-electroosmotic flow
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DOI：
10.1002/elps.201300287
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发表时间：
2014-02-01
期刊：
影响因子：
2.9
通讯作者：
Hughes, Michael P.
中科院分区：
文献类型：
--
作者：
Hoettges, Kai F.;McDonnell, Martin B.;Hughes, Michael P.
实现对环境病原体（如病毒和细菌孢子）的实时检测需要具有快速动作和合适的检测阈值的检测器。然而，大多数生物传感器的检测极限比潜在感染阈值高一个数量级或更多，限制了它们的有用性。这可以通过使用自动化样品制备技术（如预浓缩）来改善。在本文中，我们描述了使用AC电渗浓缩纳米粒子从连续流。以优化的角度穿过流动池并由1kHz信号激励的电极用于将纳米颗粒推到流动池的一侧，并从流动池的该侧提取具有高颗粒浓度的所得流。建立了一个简单的流动池中颗粒行为的模型，该模型与实验结果吻合较好。该方法表明，通过级联装置的潜在更高的浓度因子。
Achieving real-time detection of environmental pathogens such as viruses and bacterial spores requires detectors with both rapid action and a suitable detection threshold. However, most biosensors have detection limits of an order of magnitude or more above the potential infection threshold, limiting their usefulness. This can be improved through the use of automated sample preparation techniques such as preconcentration. In this paper, we describe the use of AC electroosmosis to concentrate nanoparticles from a continuous flow. Electrodes at an optimized angle across a flow cell, and energized by a 1 kHz signal, were used to push nanoparticles to one side of a flow cell, and to extract the resulting stream with a high particle concentration from that side of the flow cell. A simple model of the behavior of particles in the flow cell has been developed, which shows good agreement with experimental results. The method indicates potential for higher concentration factors through cascading devices.