Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia
Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia
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免疫相关全血细胞减少症患者外周血中 Th9 细胞增加
DOI：
10.1155/2020/6503539
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发表时间：
2020-05-05
影响因子：
4.1
通讯作者：
Fu, Rong
中科院分区：
文献类型：
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作者：
Shao, Qing;Wang, Yangyang;Fu, Rong
背景免疫相关性全血细胞减少症（Immune-related pancytoplenia，IRP）是一种由骨髓中自身抗体介导的自身免疫性疾病。辅助性T细胞9（Th 9）是近年来发现的一种新的T细胞亚群，主要表达细胞因子白细胞介素9（IL-9）发挥免疫功能。Th 9细胞与多种炎性疾病相关，但Th 9细胞在IRP中的作用仍不清楚。方法选择50例IRP患者和20例健康对照者。流式细胞术（FCM）和ELISA检测Th 9细胞的百分比。用磁珠分选CD 4+淋巴细胞，RT-PCR检测Th 9细胞相关转录因子PU. 1和BATF的mRNA表达水平。结果初治组Th 9细胞占CD 3 + CD 4+细胞的比例为2.73 ± 1.96%，明显高于缓解组（1.21 ± 0.86%）（p < 0.01）和对照组（0.68 ± 0.40%）（p < 0.001）。缓解期组明显高于对照组（P < 0.05）。未治疗组IL-9水平为183.91 ± 112.42 pg/mL，明显高于缓解组（105.96 ± 64.79 pg/mL）（p < 0.01）和对照组（56.03 ± 14.49 pg/mL）（p < 0.001）。缓解组也明显高于对照组（P < 0.01）。与血红蛋白、红细胞、白色细胞、血小板计数呈负相关，与CD 19 +B细胞、CD 5 + CD 19 +/CD 19 +B细胞百分比呈正相关。IRP组PU.1和BATF mRNA表达水平均高于对照组（p < 0.05）。结论IRP患者外周血Th 9细胞比例及血清IL-9水平升高，且与病情严重程度相关。
Background Immune-related pancytopenia (IRP) is a kind of autoimmune disease mediated by autoantibodies in bone marrow. T helper 9 (Th9) cell is a new subset of T cell discovered recently, which mainly expresses cytokine interleukin-9 (IL-9) to exert immune function. Th9 cells are associated with a variety of inflammatory diseases, but the role of Th9 cells in IRP remains unclear. Methods Fifty patients with IRP and 20 healthy controls were enrolled. The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4+ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. Results The percentage of Th9 cells in CD3+CD4+ cells was 2.73 ± 1.96% in the untreated group, which was significantly higher than those in the remission group (1.21 ± 0.86%) (p < 0.01) and the control group (0.68 ± 0.40%) (p < 0.001). And that in the remission group was significantly higher than that in the control group (p < 0.05). The level of IL-9 in the untreated group was 183.91 ± 112.42 pg/mL, which was significantly higher than that in the remission group (105.96 ± 64.79 pg/mL) (p < 0.01) and control group (56.03 ± 14.49 pg/mL) (p < 0.001). That in the remission group was also significantly higher than that in the control group (p < 0.01). They were negatively correlated with hemoglobin, red blood cell, white blood cell, and platelet counts and positively correlated with the percentage of CD19+B cells and CD5+CD19+/CD19+B cells, respectively. The mRNA expression levels of PU.1 and BATF in IRP patients were higher than those in controls (p < 0.05). Conclusions The percentage of Th9 cells in the peripheral blood and the level of IL-9 in the serum of patients with IRP were increased, which was related to the severity of the disease.