Label-free measurement of microbicidal gel thickness using low-coherence interferometry.
Label-free measurement of microbicidal gel thickness using low-coherence interferometry.
复制标题
使用低相干干涉测量法无标记测量杀菌凝胶厚度。
DOI：
10.1117/1.2192767
复制
发表时间：
2006
影响因子：
3.5
通讯作者：
Wax,Adam
中科院分区：
文献类型：
--
作者：
Braun,KellyE;Boyer,JeffreyD;Henderson,MarcusH;Katz,DavidF;Wax,Adam
Spectral-domain low-coherence interferometry (LCI) was used to measure the thickness of microbicidal gels applied to a cylindrical calibration test socket. Microbicides are topical formulations containing active ingredients targeted to inhibit specific pathogens that are currently under development for application to the epithelial lining of the lower female reproductive tract to combat sexually transmitted infections such as HIV. Understanding the deployment and drug delivery of these formulations is vital to maximizing their effectiveness. Previously,in vivomeasurements of microbicidal formulation thickness were assessed using fluorescence measurements of fluorescein-labeled gels via an optical endoscope-based device. Here we present an LCI-based device that measures the thickness of a formulation without the use of any exogenous agents by analyzing the interference pattern generated between the reflections from the front and back surface of the sample. Results are presented that validate the effectiveness and performance of the LCI measurement in a clinically relevant system as compared to an existing fluorescence-based method. The impact of the new LCI-based design onin vivomeasurements is discussed.
