The Plasmodium vivax homolog of the ookinete adhesive micronemal protein, CTRP
The Plasmodium vivax homolog of the ookinete adhesive micronemal protein, CTRP
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DOI：
10.1016/j.parint.2006.04.003
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发表时间：
2006-09-01
影响因子：
1.9
通讯作者：
Tsuboi, Takafumi
中科院分区：
文献类型：
--
作者：
Kaneko, Osamu;Templeton, Thomas J.;Tsuboi, Takafumi
疟原虫环子孢子蛋白/血小板反应蛋白相关的匿名蛋白相关蛋白（CTRP）在蚊子中肠动卵期表达，被认为是一种传播阻断疫苗的候选者。CTRP由分子胞外部分的多个血管性血友病因子A（vWA）和血小板反应蛋白I型结构域以及与肌动球蛋白机制相互作用的短酸性胞质结构域组成。作为预测CTRP内功能相关结构域的手段，我们确定了间日疟原虫萨尔和约氏疟原虫17 XL株的CTRP的核苷酸序列，并表征了疟原虫属间结构域结构和基序的保守性。序列比对表明，CTRP第1至第4 vWA结构域表现出更大的保守性，因此可能在功能上比第5和第6结构域更重要。这一点应考虑到传播阻断疫苗，包括CTRP重组亚单位的发展。为了补充以前对CTRP的细胞研究，我们进一步确定了CTRP蛋白在间日疟原虫和约氏疟原虫中的表达和细胞定位。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
The Plasmodium circumsporozoite protein/thrombospondin-related anonymous protein-related protein (CTRP) is expressed at the mosquito midgut ookinete stage and is considered to be a transmission-blocking vaccine candidate. CTRP is composed of multiple von Willebrand factor A (vWA) and thrombospondin type I domains in the extracellular portion of the molecule, and a short acidic cytoplasmic domain that interacts with the actomyosin machinery. As a means to predict functionally relevant domains within CTRP we determined the nucleotide sequences of CTRP from the Plasmodium vivax Sall and the Plasmodium yoelii 17XL strains and characterized the conservation of domain architectures and motifs across Plasmodium genera. Sequence alignments indicate that the CTRP 1st to 4th vWA domains exhibit greater conservation, and thereby are perhaps functionally more important than the 5th and 6th domains. This point should be considered for the development of a transmission-blocking vaccine that includes CTRP recombinant subunit. To complement previous cellular studies on CTRP, we further determined the expression and cellular localization of CTRP protein in R vivax and P. yoelii. (c) 2006 Elsevier Ireland Ltd. All rights reserved.