CUTANEOUS PERMEABILITY BARRIER DISRUPTION INCREASES FATTY-ACID SYNTHETIC ENZYME-ACTIVITY IN THE EPIDERMIS OF HAIRLESS MICE
CUTANEOUS PERMEABILITY BARRIER DISRUPTION INCREASES FATTY-ACID SYNTHETIC ENZYME-ACTIVITY IN THE EPIDERMIS OF HAIRLESS MICE
复制标题
DOI：
10.1111/1523-1747.ep12665893
复制
发表时间：
1995-03-01
影响因子：
6.5
通讯作者：
FEINGOLD, KR
中科院分区：
文献类型：
--
作者：
OTTEY, KA;WOOD, LC;FEINGOLD, KR
以前的研究表明，废除皮肤渗透屏障刺激表皮脂肪酸的合成，这种增加是所需的屏障修复。本研究的目的是确定这种合成增加的酶基础。通过胶带剥离的急性屏障破坏增加了表皮中乙酰辅酶A羧化酶（62%）和脂肪酸合酶（54%）的活性。类似地，通过局部丙酮处理的屏障的急性破坏增加了表皮乙酰辅酶A羧化酶（69%）和脂肪酸合酶（43%）的活性。在这两种急性模型中，通过用不渗透膜闭塞提供人工屏障防止乙酰辅酶A羧化酶和脂肪酸合成酶活性的增加，表明活性的增加依赖于经表皮水分损失的增加，并且不能归因于非特异性作用。此外，通过喂养必需脂肪酸缺乏的饮食产生的屏障的慢性破坏，也增加了表皮中的乙酰辅酶A羧化酶（127%）和脂肪酸合成酶（49%）的活性。同样，用不透膜封闭使乙酰辅酶A羧化酶和脂肪酸合成酶的活性降低到正常水平，这些结果表明，屏障破坏后表皮中发生的脂肪酸合成增加是由于表皮细胞中脂肪酸合成的协调增加。表皮乙酰辅酶A羧化酶和脂肪酸合成酶的活性。
Previous studies have shown that abrogation of the cutaneous permeability barrier stimulates epidermal fatty acid synthesis and that this increase is required for barrier repair. The purpose of the present study was to determine the enzymatic basis for this increase in synthesis, Acute barrier disruption by tape stripping increased both acetyl CoA carboxylase (62%) and fatty acid synthase (54%) activities in the epidermis, Similarly, acute disruption of the barrier by topical acetone treatment increased epidermal acetyl CoA carboxylase (69%) and fatty acid synthase (43%) activities, In both acute models, provision of an artificial barrier by occlusion with an impermeable membrane prevented the increase in acetyl CoA carboxylase and fatty acid synthase activities, indicating that the increased activity was dependent on an increase in transepidermal water loss and cannot be attributed to nonspecific effects, In addition, chronic disruption of the barrier, produced by feeding an essential-fatty-acid-deficient diet, also increased acetyl CoA carboxylase (127%) and fatty acid synthase (49%) activities in the epidermis, Again, occlusion with an impermeable membrane decreased both acetyl CoA carboxylase and fatty acid synthase activities toward normal, These results indicate that the increase in fatty acid synthesis that occurs in the epidermis after barrier disruption is due to a coordinate increase in the activities of both epidermal acetyl CoA carboxylase and fatty acid synthase.