The new live imagers MitoMM1/2 for mitochondrial visualization.
The new live imagers MitoMM1/2 for mitochondrial visualization.
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DOI：
10.1016/j.bbrc.2021.05.040
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发表时间：
2021-05
影响因子：
3.1
通讯作者：
Miwa Maeda;Mayu Suzuki;S. Takashima;Tsutomu Sasaki;K. Oh‐hashi;H. Takemori
中科院分区：
文献类型：
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作者：
Miwa Maeda;Mayu Suzuki;S. Takashima;Tsutomu Sasaki;K. Oh‐hashi;H. Takemori
线粒体是真核生物的细胞器，由内外双层膜组成，在膜间隙具有正电位（H+）。该细胞器在ATP产生和细胞凋亡中起重要作用。为了观察活细胞中的线粒体，已经开发了几种荧光染料（例如MitoTracker® [标准线粒体成像仪]或罗丹明123）。然而，这些试剂是不稳定的，并且表现出宽范围的发射光谱，从而妨碍双重染色结果。使用重组DNA技术，绿色或红色荧光蛋白（GFP或RFP）标记的蛋白质现在可用于线粒体的多色标记。在此，我们讨论了ATTO 565衍生物MitoMM 1/2的新型线粒体活体成像的发展;此外，MitoMM 1/2对膜电位敏感，耐洗涤剂，并且MitoMM 1/2的荧光与绿色荧光不重叠。
Mitochondria are eukaryotic organelles that consist of outer and inner bilayer membranes with a positive potential (H+) in the intermembrane space. This organelle plays an important role in ATP production and apoptosis. To observe the mitochondria in living cells, several fluorescent dyes (such as MitoTracker® [a standard mitochondrial imager] or rhodamine 123) have been developed. However, these reagents are unstable and exhibit a wide range of emission spectra, thereby hampering double staining results. Using recombinant DNA techniques, green or red fluorescent protein (GFP or RFP)-tagged proteins are now available for multi-color labeling of mitochondria. Here, we have discussed the development of the novel mitochondrial live imagers MitoMM1/2, derivatives of ATTO565; furthermore, MitoMM1/2 are sensitive to the membrane potential, resistant to detergents, and the fluorescence of MitoMM1/2 does not overlap with green fluorescence.