Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine
Effect of endurance training on skeletal muscle myokine expression in obese men: identification of apelin as a novel myokine
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DOI：
10.1038/ijo.2013.158
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发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Viguerie, N.
中科院分区：
文献类型：
--
作者：
Besse-Patin, A.;Montastier, E.;Viguerie, N.
背景：有研究表明，运动对代谢的益处可能是由肌因子介导的。我们研究了运动训练对人类骨骼肌中一组肌因子表达的影响。在人类myotubes.METHODS：11名肥胖的非糖尿病男性受试者参加了为期8周的耐力训练计划。通过口服葡萄糖耐量试验评估胰岛素敏感性。在训练前后采集皮下脂肪组织和股外侧肌活检样本。从脂肪组织和骨骼肌制备RNA。结果：运动训练提高了有氧能力，减少了脂肪量。训练后骨骼肌中白细胞介素6、成纤维细胞生长因子21、肌生成抑制素和鸢尾素mRNA水平无明显变化。在肌肉中发现apelin mRNA水平增加两倍，但在脂肪组织中没有。在静息状态下，没有观察到循环肌因子和脂肪因子血浆水平的变化。有趣的是，爱帕琳在原代人肌管中显著表达和分泌。爱帕琳基因表达上调环磷酸腺苷和钙，不像其他肌因子的研究。重要的是，肌肉爱帕琳mRNA水平的变化与全身胰岛素敏感性改善呈正相关。结论：总的来说，我们的数据表明，运动训练上调肌肉爱帕琳蛋白的表达在肥胖受试者。Apelin表达由运动信号通路诱导并在体外分泌于人原代肌管中，并且可能表现为具有自分泌/旁分泌作用的新型运动调节肌因子。
BACKGROUND: It has been suggested that the metabolic benefits of physical exercise could be mediated by myokines. We examined here the effect of exercise training on skeletal muscle expression of a panel of myokines in humans. Pathways regulating myokine expression were investigated in human myotubes.METHODS: Eleven obese non-diabetic male subjects were enrolled in an 8-week endurance training program. Insulin sensitivity was assessed by an oral glucose tolerance test. Subcutaneous adipose tissue and Vastus lateralis muscle biopsy samples were collected before and after training. RNAs were prepared from adipose tissue and skeletal muscle. Primary culture of myoblasts was established.RESULTS: As expected, exercise training improved aerobic capacity and decreased fat mass. No significant change in interleukin 6, fibroblast growth factor 21, myostatin (MSTN) or irisin mRNA level was found in muscle after training. A twofold increase in apelin mRNA level was found in muscle but not in adipose tissue. No change in circulating myokine and adipokine plasma levels was observed in the resting state in response to training. Interestingly, apelin was significantly expressed and secreted in primary human myotubes. Apelin gene expression was upregulated by cyclic AMP and calcium, unlike the other myokines investigated. Importantly, changes in muscle apelin mRNA levels were positively related to whole-body insulin sensitivity improvement.CONCLUSION: Collectively, our data show that exercise training upregulates muscle apelin expression in obese subjects. Apelin expression is induced by exercise signaling pathways and secreted in vitro in human primary myotubes, and may behave as a novel exercise-regulated myokine with autocrine/paracrine action.