Suppression of environmental DNA degradation in water samples associated with different storage temperature and period using benzalkonium chloride
Suppression of environmental DNA degradation in water samples associated with different storage temperature and period using benzalkonium chloride
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使用苯扎氯铵抑制与不同储存温度和时间相关的水样中的环境 DNA 降解
DOI：
10.1002/lom3.10374
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Minamoto Toshifumi
中科院分区：
文献类型：
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作者：
Takahara Teruhiko;Taguchi Junya;Yamagishi Satoshi;Doi Hideyuki;Ogata Shigeki;Yamanaka Hiroki;Minamoto Toshifumi
环境DNA（eDNA）是通过检测环境样品中所含的DNA来对目标物种进行生物监测的有用工具。然而，当估计目标物种的存在时，样品收集后eDNA降解的抑制是一个主要问题。最近，苯扎氯铵（BAC）被证明可以显着抑制淡水环境中鱼类的eDNA降解。然而，这种廉价试剂的效果尚未在其他鱼类和贝类物种中进行测试。我们检测了三种目标物种（日本蚬、花鲈和日本鳗鲡）eDNA浓度的变化。在含有添加的BAC的样品中测量eDNA。还测量了储存温度（25°C、4°C和−30°C）和储存时间（长达21 d）的影响，以确定样品降解如何受到影响。我们观察到BAC的加入是有效的抑制eDNA降解，其影响是相似的物种，但不是储存温度。除L外，其它三种植物的BAC eDNA含量均不随贮藏时间的延长而降低。在25°C和4°C下，我们的研究结果表明，BAC影响的eDNA降解普遍的各种物种栖息在淡水和微咸水。
Environmental DNA (eDNA) is a useful tool for biological monitoring of a target species by detecting DNA contained in environmental samples. However, the suppression of eDNA degradation after sample collection is a major issue when estimating the presence of a target species. Recently, benzalkonium chloride (BAC) was shown to dramatically suppress the degradation of eDNA in fish species from freshwater environments. However, the effect of this inexpensive reagent has not yet been tested in the other fish and shellfish species. We examined changes in eDNA concentrations for three target species (Corbicula japonica,Lateolabrax japonicus, andAnguilla japonica). eDNA was measured in samples containing added BAC. The effects of storage temperature (25°C, 4°C, and −30°C) and storage periods (periods up to 21 d) were also measured to determine how sample degradation was affected. We observed that BAC addition was effective in suppressing eDNA degradation, and its influence was similar among species but not storage temperatures. The concentrations of eDNA with BAC for all three species did not decrease with storage period, except forL. japonicusat 25°C and 4°C. Our results suggest that BAC affects eDNA degradation universally for a variety of species inhabiting both freshwater and brackish water.