A comparison of the daphnids Ceriodaphnia dubia and Daphnia ambigua for their utilization in routine toxicity testing in the Southeastern United States
A comparison of the daphnids Ceriodaphnia dubia and Daphnia ambigua for their utilization in routine toxicity testing in the Southeastern United States
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DOI：
10.1007/s00244-002-0116-8
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发表时间：
2003-07-01
影响因子：
4
通讯作者：
Chandler, GT
中科院分区：
文献类型：
--
作者：
Harmon, SM;Specht, WL;Chandler, GT
美国监管机构通常要求用Ceriodaphnia damina进行污水毒性测试，这种做法导致了批评，认为这种物种和测试方案往往不能反映当地的分类群或特定地点的条件。使用本地测试物种可能会产生一个更现实的模型，当地的影响，并可能尽量减少测试终点的差异，由于水质的区域差异。本研究探讨了C. dubia与Daphnia ambigua进行毒性测试。本研究确定D.该代表团指出，只要对既定的管制方案作最小的改动，就可以在实验室培养二叉水蚤，该物种的生命周期特征有利于对其他水蚤采用传统的急性和慢性水生毒性测试方法。急性毒性试验表明，D.青虫对某些毒物（氯化钠、硫酸铜和十二烷基硫酸钠）不太敏感，但对其他毒物（毒死蜱）更敏感。硫酸铜和氯化钠的慢性试验导致D的EC（50）较低。与两种化合物的ambigia繁殖。当暴露于低碱度，低pH值流沃茨典型的许多美国东南部流域，C。dubia表现出显着的生殖抑制，在两个测试的三个溪流，而D。ambigua没有慢性效应。这些结果表明D. ambigua可以作为C. dubia作为这些类型的接收流的毒性指示物种。
U.S. regulatory agencies commonly require effluent toxicity testing with Ceriodaphnia dubia-a practice that has led to the criticism that this species and test protocol often does not reflect local taxa or site-specific conditions. Using an indigenous test species may produce a more realistic model of local effects and may minimize test endpoint variance due to regional differences in water quality. This study addressed the substitution of C. dubia with Daphnia ambigua for toxicity testing in the southeastern United States. This investigation determined that D. ambigua could be laboratory cultured with only minimal changes to established regulatory protocol and that the life-cycle characteristics of this species were conducive to traditional acute and chronic aquatic toxicity test methods used with other daphnids. Acute toxicity tests showed that D. ambigua was less sensitive to some toxicants (sodium chloride, copper sulfate, and sodium lauryl sulfate) but more sensitive to others (chlorpyrifos). Chronic tests with copper sulfate and sodium chloride resulted in lower EC(50)s for D. ambigua reproduction with both compounds. When exposed to low-alkalinity, low-pH stream waters typical of many southeastern United States watersheds, C. dubia demonstrated a significant reproductive depression in two of three streams tested, whereas D. ambigua experienced no chronic effect. These results suggest that D. ambigua may serve as a suitable surrogate for C. dubia as an toxicity indicator species in these types of receiving streams.