Annexin A2 localizes to the basal epithelial layer and is down-regulated in dysplasia and head and neck squamous cell carcinoma
Annexin A2 localizes to the basal epithelial layer and is down-regulated in dysplasia and head and neck squamous cell carcinoma
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DOI：
10.1016/j.canlet.2007.12.029
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发表时间：
2008-05-08
期刊：
影响因子：
9.7
通讯作者：
Pilar Fernandez, M.
中科院分区：
文献类型：
--
作者：
Pena-Alonso, Emma;Pablo Rodrigo, Juan;Pilar Fernandez, M.
膜联蛋白A2是一种在细胞膜生理学中具有重要作用的高表达基因，并且在癌症中经常失调。本研究的目的是确定膜联蛋白A2蛋白在头颈部鳞状细胞癌中的表达模式及其预后意义。我们评估了定量变化和膜联蛋白A2的mRNA和蛋白质的表达在正常和病变组织的定性分布，免疫组织化学，免疫荧光和原位杂交。膜联蛋白A2的表达仅限于正常上皮的基底细胞和基底上细胞，并且蛋白质细胞定位在细胞膜上。表达水平与组织病理学分级相关，在中分化和低分化肿瘤中表现出显著抑制。我们的结论是，膜联蛋白A2表现出一种特征性的表达模式，不同于其他膜联蛋白，并提示细胞特异性的功能作用。膜联蛋白A2在低分化肿瘤和发育不良组织中的显著减少预期导致旨在协调膜信号传导酶复合物、肌动蛋白聚合和细胞外基质蛋白水解的功能丧失。表型的后果可能会变得明显的改变上皮组织的生长和重塑与继发性影响肿瘤的发展，进展和转移。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Annexin A2 is a highly expressed gene with important roles in cell membrane physiology and is frequently dysregulated in cancer. The objective Of this Study was to determine the pattern of expression and prognostic significance of annexin A2 protein in head and neck squamous cell carcinoma. We assessed both quantitative changes and qualitative distribution of annexin A2 mRNA and protein expression in normal and diseased tissues by immunohistochemistry, immunofluorescence and in situ hybridization. Annexin A2 expression was confined to the basal and suprabasal cells of normal epithelium and the protein cellular location was consistently observed at the cell membrane. Expression levels correlated with histopathological grade, showing significant suppression in moderately and poorly differentiated tumors. We conclude that annexin A2 exhibits a characteristic pattern of expression, distinct from other annexins and suggestive of a cell-specific functional role. The marked reduction of annexin A2 in poorly differentiated tumors and dysplastic tissue is expected to result in a loss of function aimed at the coordination of membrane signalling enzyme complexes, actin polymerization and extracellular matrix proteolysis. The phenotypic consequences may become manifest in an alteration of epithelial tissue growth and remodelling with secondary influence on tumour development, progression and metastasis. (C) 2007 Elsevier Ireland Ltd. All rights reserved.