Gamma knife radiosurgery targeting protocols for the experiments with small animals
Gamma knife radiosurgery targeting protocols for the experiments with small animals
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DOI：
10.1159/000099071
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Kouyama, Nobuo
中科院分区：
文献类型：
--
作者：
Tokumaru, Osamu;Hayashi, Motohiro;Kouyama, Nobuo
背景/目标：通过伽玛刀（GK）照射操纵脑功能将在临床和实验神经学中有许多应用。方法：通过对大鼠自由活动行为的监测，观察单侧照射后纹状体脑功能的变化。阿扑吗啡试验可以检测多巴胺能功能的不平衡，采用我们的行为跟踪系统对阿扑吗啡试验的自发活动和旋转进行索引。使用连续切片探索坏死病变的空间分布，并假设其代表GK靶点的真实的病灶。结果：不同的行为改变对应于基底节不同区域的病变的精确位置。位移的照射部位在前内侧方向增加自发活动，和后外侧移位引起盘旋行为的阿扑吗啡测试。结论：准确定位的目标是至关重要的实验GK照射局部集中在一个小的大脑，如大鼠的域。在这里，我们提出了一个协议，用于转换的“预期”的重点，基于脑地图坐标，到一个“计划”的重点与Regis-Valliccioni立体定向框架的MR成像坐标系。版权所有（c）2007 S. Karger AG，巴塞尔
Background/Aims: Manipulation of brain functions via Gamma Knife (GK) irradiation would have numerous applications in clinical and experimental neurology. Methods: Alteration of brain functions in the unilaterally irradiated striatum was indexed through monitoring freely moving rat behaviors. Spontaneous activity and rotations on the apomorphine test, which can detect dopaminergic function imbalance, were indexed employing our behavior tracking system. The spatial distribution of necrotic lesions was explored using serial sections, and was assumed to represent the real foci of the GK target. Results: Distinct behavioral alterations corresponded to the precise locations of the lesions in various areas of the basal ganglia. Displacement of the irradiation sites in the anteromedial direction increased spontaneous activity, and posterolateral shift provoked circling behavior on the apomorphine test. Conclusion: Accurate positioning of the target is crucial for experimental GK irradiation locally focused on domains of a small brain such as that of the rat. Here, we propose a protocol for converting the 'intended' focus, based on brain map coordinates, to a 'planned' focus on the MR imaging coordinate system with the Regis-Valliccioni stereotactic frame. Copyright (c) 2007 S. Karger AG, Basel