DIFFERENTIAL REGULATION OF CYCLIC-GMP LEVELS IN THE FRONTAL-CORTEX AND THE CEREBELLUM OF ANESTHETIZED RATS BY NITRIC-OXIDE - AN IN-VIVO MICRODIALYSIS STUDY
DIFFERENTIAL REGULATION OF CYCLIC-GMP LEVELS IN THE FRONTAL-CORTEX AND THE CEREBELLUM OF ANESTHETIZED RATS BY NITRIC-OXIDE - AN IN-VIVO MICRODIALYSIS STUDY
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DOI：
10.1016/0006-8993(94)90517-7
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发表时间：
1994-12-30
期刊：
影响因子：
2.9
通讯作者：
AIRAKSINEN, MM
中科院分区：
文献类型：
--
作者：
LAITINEN, JT;LAITINEN, KSM;AIRAKSINEN, MM
采用微透析结合放射免疫分析法，对麻醉大鼠额叶皮层和小脑细胞外环鸟苷酸（cGMP）水平进行了动态监测。在整个灌注期间，基础cGMP释放保持恒定，在额叶皮质中约为2 fmol/30 min，在小脑中约为4 fmol/30 min。一氧化氮（NO）供体硝普钠（SNP）刺激cGMP释放短暂的两个区域。然而，最大反应是3倍，在额叶皮质（获得5 μ M SNP），但90倍，在小脑（获得1 mM SNP）。灌注NO合酶（NOS）抑制剂N-G-硝基-L-精氨酸甲酯（L-NAME）抑制小脑cGMP释放74%，表明NO是小脑中基础cGMP水平的主要调节剂。相反，L-NAME在额叶皮层中没有表现出效力，这表明鸟苷酸环化酶的其他激活剂可以调节体内基底皮层cGMP水平。
A microdialysis method combined with a sensitive radioimmunoassay was used to monitor extracellular cyclic GMP (cGMP) levels in the frontal cortex and the cerebellum of anesthetized rats in vivo. Basal cGMP release remained constant throughout the perfusion period and was approximate to 2 fmol/30 min in the frontal cortex and approximate to 4 fmol/30 min in the cerebellum. The nitric oxide (NO) donor sodium nitroprusside (SNP) stimulated cGMP release transiently in both regions. However, the maximal response was 3-fold in the frontal cortex (obtained with 5 mu M SNP) but 90-fold in the cerebellum (obtained with 1 mM SNP). Perfusion with the NO synthase (NOS) inhibitor N-G-nitro-L-arginine methyl ester (L-NAME) suppressed cerebellar cGMP release by 74% indicating that NO is the major regulator of basal cGMP levels in the cerebellum. Quite opposite, L-NAME exhibited no potency in the frontal cortex suggesting that other activators of guanylyl cyclase may regulate basal cortical cGMP levels in vivo.