Th1/Th2 and regulatory cytokines in adults with otitis media with effusion
Th1/Th2 and regulatory cytokines in adults with otitis media with effusion
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DOI：
10.1097/01.mao.0000224087.93096.4d
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发表时间：
2006-12-01
影响因子：
2.1
通讯作者：
Nishizaki, Kazunori
中科院分区：
文献类型：
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作者：
Kariya, Shin;Okano, Mitsuhiro;Nishizaki, Kazunori
目的：渗出性中耳炎是最常见、最难治的耳部疾病之一。然而，Th1、Th2 和免疫调节细胞因子在成年患者疾病发病机制中的作用仍有待确定。本研究的目的是揭示成人中耳积液 (MEE) 中的细胞因子表达，并根据是否存在过敏性鼻炎和积液类型来比较其概况。研究设计：一项前瞻性对照临床研究。患者：从 80 名成人受试者中收集 MEE。采用酶联免疫吸附法测定MEE中白细胞介素(IL)-2、IL-4、IL-5、IL-10、IL-12和干扰素(IFN)-γ的浓度。结果：MEE中检测到IL-2、IL-4、IL-5、IL-10、IL-12和IFN-gamma，60例(75.0%)、33例(41.3%)、分别有 42 个（52.5%）、14 个（17.5%）、80 个（100%）和 66 个（82.5%）样本。这些细胞因子中，只有IL-4的浓度在变应性鼻炎阳性组中显着高于变应性鼻炎阴性组。另一方面，无论是否存在过敏性鼻炎，都检测到IL-2、IL-12和IFN-γ，并且这些细胞因子的浓度彼此相关。还检测了IL-4和IL-5浓度之间的相关性。此外，与浆液型积液相比，粘液型MEE的发生率和MEE中IL-10的浓度均显着升高。结论：无论过敏状态如何，IL-12可能通过影响IL-2和IFN-γ的产生而在积液性中耳炎的发病机制中发挥关键作用。此外，IL-4可能对成人过敏性鼻炎的免疫状况有一定影响。 IL-10 可能会影响 MEE 的粘度。
Objective: Otitis media with effusion is one of the most common and intractable ear diseases. However, the role of Th1, Th2, and immunoregulatory cytokines on the pathogenesis of the disease in adult patients remains to be determined. The aim of this study is to disclose the cytokine expression in middle ear effusions (MEEs) in adults and to compare the profile on the basis of the presence of allergic rhinitis and the type of effusions.Study Design: A prospective controlled clinical study.Patients: MEEs were collected from 80 adult subjects. The concentration of interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12, and interferon (IFN)-gamma in MEEs were determined by using enzyme-linked immunosorbent assay.Results: IL-2, IL-4, IL-5, IL-10, IL-12, and IFN-gamma in MEEs were detected in 60 (75.0%), 33 (41.3%), 42 (52.5%), 14 (17.5%), 80 (100%), and 66 (82.5%) samples, respectively. Among these cytokines, only the concentration of IL-4 in the allergic rhinitis-positive group was significantly higher than that in the allergic rhinitis-negative group. On the other hand, IL-2, IL-12, and IFN-gamma were detected, regardless of the presence of allergic rhinitis, and the concentration of these cytokines correlated with each other. The correlation between the concentration of IL-4 and IL-5 was also detected. In addition, both the incidence rate and the concentration of IL-10 in MEEs were significantly higher in the mucoid type compared with those in the serous type effusions.Conclusion: Regardless of allergic status, IL-12 may play a critical role in the pathogenesis of otitis media with effusion by affecting the production of IL-2 and IFN-gamma. In addition, IL-4 may have some impact on the immunologic condition in adults with allergic rhinitis. IL-10 potentially affects the viscosity of MEEs.