Analytical strategies to assess the functional metabolome of vitamin E.
Analytical strategies to assess the functional metabolome of vitamin E.
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DOI：
10.1016/j.jpba.2016.01.056
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发表时间：
2016-05
影响因子：
3.4
通讯作者：
P. Torquato;Orsola Ripa;D. Giusepponi;R. Galarini;D. Bartolini;Maria Wallert;R. Pellegrino;G. Cruciani-G
中科院分区：
文献类型：
--
作者：
P. Torquato;Orsola Ripa;D. Giusepponi;R. Galarini;D. Bartolini;Maria Wallert;R. Pellegrino;G. Cruciani-G
在发现90多年和发表了数千篇论文之后，维生素E（生育酚和生育三烯酚）的生理作用仍然没有完全阐明。在过去的几十年里，这种维生素的酶代谢已经成为一个令人兴奋的研究课题。它的阐明为解释这类分子的生物学功能开辟了新的视野。一些生理代谢物的特定性质的鉴定和先进的分析技术的定义，以评估这种维生素在体内的人体代谢组，已经铺平了道路，一系列的假设功能的影响，这种代谢可能远远超出其分解代谢的作用。本综述收集了用于评估人体以及其他模型系统中维生素E的状态和代谢的最相关分析策略的现有信息。一个特别的重点是致力于用于测量维生素E代谢物的分析方法，特别是长链代谢物，在生物液体和组织。初步信息的一个新的LC-APCI-MS/MS方法来测量这些代谢物在人血清中的报告。
After more than 90 years from its discovery and thousands of papers published, the physiological roles of vitamin E (tocopherols and tocotrienols) are still not fully clarified. In the last few decades, the enzymatic metabolism of this vitamin has represented a stimulating subject of research. Its elucidation has opened up new horizons to the interpretation of the biological function of that class of molecules. The identification of specific properties for some of the physiological metabolites and the definition of advanced analytical techniques to assess the human metabolome of this vitaminin vivo, have paved the way to a series of hypotheses on the functional implications that this metabolism may have far beyond its catabolic role. The present review collects the available information on the most relevant analytical strategies employed to assess the status and metabolism of vitamin E in humans as well as in other model systems. A particular focus is dedicated to the analytical methods used to measure vitamin E metabolites, and particularly long-chain metabolites, in biological fluids and tissues. Preliminary information on a new LC-APCI-MS/MS method to measure these metabolites in human serum is reported.