Comment on Effective Temperature and Structural Rearrangement in Trapped Ion Mobility Spectrometry: TIMS Enables Native Mass Spectrometry Applications.
Comment on Effective Temperature and Structural Rearrangement in Trapped Ion Mobility Spectrometry: TIMS Enables Native Mass Spectrometry Applications.
复制标题
对俘获离子淌度光谱中有效温度和结构重排的评论：TIMS 实现了原生质谱应用。
作者：
C. Bleiholder;Fanny C. Liu;M. Chai
离子迁移率光谱/质谱学作为生物化学中的一种结构工具，依赖于天然溶液结构在测量时间尺度内是亚稳定的这一概念。因此，关键的问题是，在离子迁移率光谱仪中，离子从电场中获得多少热？Morsa等人。研究了囚禁离子迁移率光谱仪(TIMS)中的离子加热，声称大量的离子加热是TIMS固有的，并质疑TIMS保留生物相关结构的能力。然而，Morsa等人在已知为热的条件下测量了有效离子温度，然后声称观察到的加热是TIMS方法的固有特性。因此，在本评论中，我们澄清(A)已报道的离子加热不是TIMS固有的，以及(B)TIMS使自然质谱学应用成为可能。
Ion mobility spectrometry/mass spectrometry as a structural tool in biochemistry relies on the notion that native solution structures are metastable within the measurement timescale. Ergo, the critical question is, how much "heat" do ions take up from electric fields in an ion mobility spectrometer? Morsa et al. investigated ion heating in a trapped ion mobility spectrometer (TIMS), claiming that substantial ion heating is intrinsic to TIMS and questioning the ability of TIMS to retain biologically relevant structures. Morsa et al., however, measure the effective ion temperature under conditions that are known to be "hot" and then claim that the observed heating is an intrinsic property of the TIMS method. In this Comment, we thus clarify (a) that the reported ion heating is not intrinsic to TIMS and (b) that TIMS enables native mass spectrometry applications.
影响因子：
7.4
作者：
Zhang X;Garimella SV;Prost SA;Webb IK;Chen TC;Tang K;Tolmachev AV;Norheim RV;Baker ES;Anderson GA;Ibrahim YM;Smith RD
通讯作者：
Smith RD
DOI：
10.1007/s13361-019-02200-y
发表时间：
2019-07-01
影响因子：
3.2
作者：
Kirk, Samuel R.;Liu, Fanny C.;Bleiholder, Christian
通讯作者：
Bleiholder, Christian
影响因子：
1.8
作者：
May JC;Jurneczko E;Stow SM;Kratochvil I;Kalkhof S;McLean JA
通讯作者：
McLean JA