Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays.
Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays.
复制标题
使用小体积分支 DNA 测定对血浆中人类免疫缺陷病毒 1 型 RNA 水平进行定量。
DOI:
10.1128/jcm.36.7.2096-2098.1998
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发表时间:
1998
影响因子:
9.4
通讯作者:
Yogev,R
中科院分区:
文献类型:
--
作者:
Yeghiazarian,T;Zhao,Y;Read,SE;Kabat,W;Li,X;Hamren,SJ;Sheridan,PJ;Wilber,JC;Chernoff,DN;Yogev,R
We have developed small-volume (50 or 250 μl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.