Cloning of the cDNA and the genomic DNA of the mouse angiotensin II type 2 receptor.
Cloning of the cDNA and the genomic DNA of the mouse angiotensin II type 2 receptor.
复制标题
小鼠血管紧张素 II 2 型受体 cDNA 和基因组 DNA 的克隆。
DOI：
10.1016/0005-2736(94)90072-8
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Inagami,T
中科院分区：
文献类型：
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作者：
Ichiki,T;Herold,CL;Kambayashi,Y;Bardhan,S;Inagami,T
在血管紧张素II受体的两种主要亚型，1型（AT1）和2型（AT2）中，对AT2的结构和特征知之甚少。我们从小鼠胎儿cDNA文库中克隆了一个小鼠AT2cDNA，从129SV小鼠基因组DNA文库中克隆了一个小鼠at2dna。从小鼠cDNA克隆中推导出小鼠AT2（363个残基）的氨基酸序列，显示出7个跨膜结构域。小鼠AT2与小鼠at1氨基酸同源性为37%，与大鼠AT2氨基酸同源性为98%。基因组DNA （4.4 kb）包含3个外显子和2个内含子，整个编码区包含在第3个外显子中。
Of the two major isoforms of the angiotensin II receptors, type 1 (AT1) and type 2 (AT2), little is known about the structure and features of AT2. We cloned a mouse AT2cDNA from a mouse fetus cDNA library and an AT2genomic DNA from a 129SV mouse genomic DNA library. The amino acid sequence of the mouse AT2(363 residues) deduced from a mouse cDNA clone showed seven membrane-spanning domains. Amino acid identity of the mouse AT2with mouse AT1is 37%, and 98% with rat AT2. The genomic DNA (4.4 kb) contained three exons and two introns and the entire coding region was contained in the third exon.