P. aeruginosa controls both C. elegans attraction and pathogenesis by regulating nitrogen assimilation.
P. aeruginosa controls both C. elegans attraction and pathogenesis by regulating nitrogen assimilation.
复制标题
铜绿假单胞菌通过调节氮同化来控制线虫的吸引和发病机制。
DOI：
10.1101/2023.11.29.569279
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Gitai,Zemer
中科院分区：
文献类型：
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作者：
Marogi,JacobG;Murphy,ColeenT;Myhrvold,Cameron;Gitai,Zemer
检测化学信号对于识别食物来源和避免有害物质非常重要。像大多数动物一样，C.线虫利用嗅觉对其主要食物来源细菌进行趋化。然而，人们对控制 C 的细菌化合物知之甚少。线虫对细菌的吸引力以及这些化合物对细菌的生理重要性。在这里，我们通过研究病原体铜绿假单胞菌中的小 RNA P11 的功能来解决这些问题，之前已证明这种小 RNA 可以介导习得性病原体回避。我们发现这种 RNA 也会影响未经训练的 C 的吸引力。 eleganstopP.铜绿假单胞菌通过控制氨的产生来实现这一点，氨是氮同化过程中产生的挥发性气味剂。我们解开了 P 的复杂调节。铜绿假单胞菌氮同化，由涉及环境硝酸盐、传感器蛋白和 P11 的伙伴转换机制介导。除了调解之外C.线虫吸引、氮同化对于 C 期间的细菌适应性和发病机制很重要。线虫感染。铜绿假单胞菌。这些研究将氨定义为跨界信号传导的主要介质，揭示了氮同化对细菌和宿主生物体的生理重要性，并强调了细菌代谢途径如何在不同环境下有益或有害宿主。
Detecting chemical signals is important for identifying food sources and avoiding harmful agents. Like most animals,C. elegansuse olfaction to chemotax towards their main food source, bacteria. However, little is known about the bacterial compounds governingC. elegansattraction to bacteria and the physiological importance of these compounds to bacteria. Here, we address these questions by investigating the function of a small RNA, P11, in the pathogen,Pseudomonas aeruginosa,that was previously shown to mediate learned pathogen avoidance. We discovered that this RNA also affects the attraction of untrainedC. eleganstoP. aeruginosaand does so by controlling production of ammonia, a volatile odorant produced during nitrogen assimilation. We untangle the complex regulation ofP. aeruginosanitrogen assimilation, which is mediated by a partner-switching mechanism involving environmental nitrates, sensor proteins, and P11. In addition to mediatingC. elegansattraction, nitrogen assimilation is important for bacterial fitness and pathogenesis duringC. elegansinfection byP. aeruginosa. These studies define ammonia as a major mediator of trans-kingdom signaling, reveal the physiological importance of nitrogen assimilation for both bacteria and host organisms, and highlight how a bacterial metabolic pathway can either benefit or harm a host in different contexts.