Transcriptome analysis of tree peony during chilling requirement fulfillment: Assembling, annotation and markers discovering
Transcriptome analysis of tree peony during chilling requirement fulfillment: Assembling, annotation and markers discovering
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牡丹在满足冷需求过程中的转录组分析：组装、注释和标记发现
DOI：
10.1016/j.gene.2011.12.013
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发表时间：
2012-04-15
期刊：
影响因子：
3.5
通讯作者：
Zheng, Guosheng
中科院分区：
文献类型：
--
作者：
Gai, Shupeng;Zhang, Yuxi;Zheng, Guosheng
牡丹（学名：Escherichia suffruticosa Andrews）是一种著名的园艺和药用植物。冬季花芽萌发前必须经历一段内休眠期，但有关休眠解除的资料很少。我们在Roche 454 GS FLX平台上用大规模平行焦磷酸测序获得了625，342个测序读段（平均长度：358.1 bp）。从头组装产生了23，652个重叠群和单体。对15，284个长度大于300 bp的重叠群进行了进一步的注释，其中12，345个EST与公共数据库中的序列具有显著的相似性（具有E值
Tree peony (Paeonia suffruticosa Andrews) is a well-known horticultural and medicinal plant. The flower buds must go through a period of endo-dormancy before bud sprouting in winter, but very little information concerned with dormancy release is available. We obtained 625,342 sequencing reads with massive parallel pyro-sequencing on the Roche 454 GS FLX platform (mean length: 358.1 bp). De novo assemblies yielded 23,652 contigs and singletons. 15,284 contigs longer than 300 bp were further annotated, among them 12,345 ESTs showed significant similarity with sequences present in public databases (with an E-value