An ultra acid stable reversed stationary phase.
An ultra acid stable reversed stationary phase.
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超酸稳定的反相固定相。
DOI：
10.1021/ja036339d
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发表时间：
2003
影响因子：
15
通讯作者：
Carr,PeterW
中科院分区：
文献类型：
--
作者：
Trammell,BrianC;Ma,Lianjia;Luo,Hao;Hillmyer,MarcA;Carr,PeterW
我们报告了一种新的反相液相色谱 (RPLC) 相，使用二甲基（乙基苯基氯甲基）氯硅烷 (1)、低聚聚苯乙烯 (PS) 和辛基苯 (C8)，具有显着的酸稳定性。此相 Si-1-PS-C8 是使用二氧化硅改性工艺和 Friedel−Crafts 烷基化化学制备的。在高侵蚀性流动相条件下，Si-1-PS-C8 表现出卓越的稳定性，在 pH = 0.5 和 150 °C 下经过 1400 个柱体积后，保留因子 (k‘) 仅略有下降。使用 Si-1-PS-C8，各种碱性溶质的峰形是对称的。使用扫描电子显微镜观察到二氧化硅颗粒高度交联涂层的证据。与迄今为止报道的所有其他 RPLC 固定相相比，该固定相具有无与伦比的稳定性。
We report a new reversed phase liquid chromatography (RPLC) phase with remarkable acid stability using dimethyl(ethylphenylchloromethyl)chlorosilane (1), oligomeric polystyrene (PS), and octylbenzene (C8). This phase Si-1-PS-C8was prepared using silica modification processes and Friedel−Crafts alkylation chemistry. Under highly aggressive mobile phase conditions, Si-1-PS-C8exhibited remarkable stability as evinced by only minimal reduction in retention factor (k‘) after 1400 column volumes at pH = 0.5 and 150 °C. The peak shapes for a variety of basic solutes were symmetric using Si-1-PS-C8. Evidence for a highly cross-linked coating of the silica particles was observed using scanning electron microscopy. The remarkable stability of this phase is unparalleled as compared to all other RPLC phases reported to date.