Circ-TCF4.85 silencing inhibits cancer progression through microRNA-486-5p-targeted inhibition of ABCF2 in hepatocellular carcinoma (Retracted Article)
Circ-TCF4.85 silencing inhibits cancer progression through microRNA-486-5p-targeted inhibition of ABCF2 in hepatocellular carcinoma (Retracted Article)
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DOI：
10.1002/1878-0261.12603
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发表时间：
2020-01-10
影响因子：
6.6
通讯作者：
Zhou, Fan
中科院分区：
文献类型：
--
作者：
Gao, Jun;Dai, Chao;Zhou, Fan
本研究旨在探讨环状RNA CIRC-TCF4.85及其下游靶基因microRNA-486-5p(miR-486-5p)在肝细胞癌发生发展中的作用。CIRC-TCF4.85在肝细胞癌组织中高表达。接下来，我们发现沉默CIRC-TCF4.85抑制了肝癌细胞的增殖、侵袭和迁移，同时促进了细胞凋亡。此外，生物素偶联探针下拉和miRNA捕获实验以及荧光原位杂交证实CIRC-TCF4.85能够与miR-486-5p结合。在挽救实验中，miR-486-5p有可能消除CIRC-TCF4.85基因敲除对肝癌的抑瘤作用。此外，miR-486-5p以ABCF2基因为靶点，受CIRC-TCF4.85正向调控。最后，裸鼠皮下注射转SI-CIRC-TCF4.85的肝癌细胞后，体内移植瘤的形成受到抑制。综上所述，我们的结果表明，沉默CIRC-TCF4.85通过miR-486-5P靶向抑制ABCF2来抑制肝癌的进展。
The current study aimed to explore the role of the circular RNA circ-TCF4.85 and its downstream target microRNA-486-5p (miR-486-5p) in hepatocellular carcinoma (HCC) development. Circ-TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ-TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin-coupled probe pull-down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ-TCF4.85 could bind to miR-486-5p. In rescue experiments, miR-486-5p had the potential to eliminate the tumor-suppressive effects of circ-TCF4.85 knockdown in HCC. Moreover, miR-486-5p was shown to target ABCF2 gene, which was positively regulated by circ-TCF4.85. Finally, nude mice subcutaneously injected with si-circ-TCF4.85-transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ-TCF4.85 suppresses HCC progression via miR-486-5p-targeted inhibition of ABCF2.