Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering
Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering
复制标题
使用表面增强拉曼散射监测活细胞中的内源硫化氢
DOI：
10.1002/anie.201505025
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发表时间：
2015-10-19
影响因子：
16.6
通讯作者：
Tian, He
中科院分区：
文献类型：
--
作者：
Li, Da-Wei;Qu, Lu-Lu;Tian, He
硫化氢（H2S）已成为一种重要的气体递质在不同的生理过程中，虽然它的作用的许多方面仍然不清楚，部分原因是缺乏强大的分析方法。本文报道了一种新型的表面增强拉曼散射（Sers）纳米传感器--4-乙酰氨基苯磺酰叠氮功能化金纳米粒子（AuNPs/4-AA），用于检测活细胞中内源性H2S。利用H_2S与4-AA在AuNPs上反应产生的AuNPs/4-AA的Sers光谱变化完成检测。Sers纳米传感器对H2S具有高选择性。此外，AuNPs/4-AA响应H2S在1分钟内与0.1 μ m的灵敏度水平。特别是，我们的Sers方法可以用来监测内源性H2S在活胶质瘤细胞中产生的，证明其在涉及H2S的病理生理途径的研究中的巨大前景。
Hydrogen sulfide (H2S) has emerged as an important gasotransmitter in diverse physiological processes, although many aspects of its roles remain unclear, partly owing to a lack of robust analytical methods. Herein we report a novel surface-enhanced Raman scattering (SERS) nano-sensor, 4-acetamidobenzenesulfonyl azide-functionalized gold nanoparticles (AuNPs/4-AA), for detecting the endogenous H2S in living cells. The detection is accomplished with SERS spectrum changes of AuNPs/4-AA resulting from the reaction of H2S with 4-AA on AuNPs. The SERS nanosensor exhibits high selectivity toward H2S. Furthermore, AuNPs/4-AA responds to H2S within 1 min with a 0.1 mu m level of sensitivity. In particular, our SERS method can be utilized to monitor the endogenous H2S generated in living glioma cells, demonstrating its great promise in studies of pathophysiological pathways involving H2S.