Heterogeneous IgE reactivities to <i>Staphylococcus pseudintermedius</i> strains in dogs with atopic dermatitis, and the identification of DM13-domain-containing protein as a bacterial IgE-reactive molecule
Heterogeneous IgE reactivities to <i>Staphylococcus pseudintermedius</i> strains in dogs with atopic dermatitis, and the identification of DM13-domain-containing protein as a bacterial IgE-reactive molecule
复制标题
特应性皮炎犬体内对<i>伪中间葡萄球菌</i>菌株的异质 IgE 反应性，以及含 DM13 结构域的蛋白质作为细菌 IgE 反应性分子的鉴定
DOI：
10.1093/femsle/fnac019
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发表时间：
2022
影响因子：
2.1
通讯作者：
Sakaguchi Ma
中科院分区：
文献类型：
--
作者：
Takemura-Uchiyama Iyo;Tsurui Hiroki;Shimakura Hidekatsu;Nasukawa Tadahiro;Imanishi Ichiro;Uchiyama Jumpei;Fukuyama Tomoki;Sakamoto Shuji;Morisawa Keiko;Fujimura Masato;Murakami Hironobu;Kanamaru Shuji;Kurokawa Kenji;Kawamoto Keiko;Iyori Keita;Sakaguchi Ma
假间质葡萄球菌是引起犬皮肤感染的主要病原体之一。在犬特应性皮炎（AD）中，s。假中间体存在于受影响的皮肤部位。据报道，由于这种细菌的特异性IgE增加，S。假媒介病可能加重犬AD的严重程度。在本研究中，IgE对各种。伪中间菌和s。pseudintermediuswere调查。首先，对8株s。采用141份AD犬血清，对s。假中间体显示10-63%的IgE反应性。这与基于AD患者金黄色葡萄球菌克隆性概念的预期结果不同。此外，根据western blot分析，有4种以上的蛋白对IgE有反应。随后，在~ 15 kDa处对常见ige反应蛋白进行分析，证实含有dm13结构域的蛋白在AD犬中具有反应性，这与任何s都不一致。aureusIgE-reactive分子。考虑到这些,S。假性中间病可能会加重狗的AD严重程度，与s的情况略有不同。金黄色葡萄球菌人AD。
Staphylococcus pseudintermediusis one of the major pathogens causing canine skin infection. In canine atopic dermatitis (AD), heterogeneous strains ofS. pseudintermediusreside on the affected skin site. Because an increase in specific IgE to this bacterium has been reported,S. pseudintermediusis likely to exacerbate the severity of canine AD. In this study, the IgE reactivities to variousS. pseudintermediusstrains and the IgE-reactive molecules ofS. pseudintermediuswere investigated. First, examining the IgE reactivities to eight strains ofS. pseudintermediususing 141 sera of AD dogs, strain variation ofS. pseudintermediusshowed 10–63% of the IgE reactivities. This is different from the expected result based on the concept ofStaphylococcus aureusclonality in AD patients. Moreover, according to the western blot analysis, there were more than four proteins reactive to IgE. Subsequently, the analysis of the common IgE-reactive protein at ∼15 kDa confirmed that the DM13-domain-containing protein was reactive in AD dogs, which is not coincident with anyS. aureusIgE-reactive molecules. Considering these,S. pseudintermediusis likely to exacerbate AD severity in dogs, slightly different from the case ofS. aureusin human AD.