Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity
Fatty Acid Binding Protein 7 is Involved in the Proliferation of Reactive Astrocytes, but not in Cell Migration and Polarity
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DOI：
10.1267/ahc.20001
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发表时间：
2020-01-01
影响因子：
2.4
通讯作者：
Kagawa, Yoshiteru
中科院分区：
文献类型：
--
作者：
Hara, Tomonori;Umaru, Banlanjo Abdulaziz;Kagawa, Yoshiteru
反应性神经胶质增生是一种防御机制，可最大限度地减少和修复中枢神经系统损伤后的初始损伤，其特征是星形胶质细胞反应性和增殖增加，神经胶质纤维酸性蛋白（GFAP）表达增强和细胞肥大。在大脑发育过程中，脂肪酸结合蛋白 7 (FABP7) 在多种类型的神经胶质细胞中大量表达，例如星形胶质细胞和少突胶质细胞前体细胞，并且在刺伤后，FABP7 阳性星形胶质细胞在小鼠皮质中显着增加。然而，FABP7 在神经胶质增生中的功能意义仍不清楚。在本研究中，我们使用原代培养的星形胶质细胞体外划伤损伤模型检查了 FABP7 介导的神经胶质增生调节机制。 Western blotting显示，与完整的星形胶质细胞相比，划伤边缘的星形胶质细胞中FABP7表达显着增加。通过监测损伤区域的占用情况，48小时后，与WT星形胶质细胞相比，FAB7-KO星形胶质细胞显示出较慢的增殖率，这通过BrdU免疫染色得到证实。 FABP-KO 和 WT 之间的细胞迁移和反应性星形胶质细胞的极性没有差异。总之，我们的数据表明 FABP7 在 CNS 损伤情况下反应性星形胶质细胞的增殖中发挥重要作用。
Reactive gliosis is a defense mechanism to minimize and repair the initial damage after CNS injuries that is characterized by increases in astrocytic reactivity and proliferation, with enhanced expression of glial fibrillary acidic protein (GFAP) and cellular hypertrophy. Fatty acid binding protein 7 (FABP7) is abundantly expressed in several types of glial cells, such as astrocytes and oligodendrocyte precursor cells, during brain development and FABP7-positive astrocytes have been shown to be significantly increased in the mouse cortex after a stab injury. However, the functional significance of FABP7 in gliosis remains unclear. In the present study, we examined the mechanism of FABP7-mediated regulation of gliosis using an in vitro scratch-injury model using primary cultured astrocytes. Western blotting showed that FABP7 expression was increased significantly in scratch wounded astrocytes at the edge of the injury compared with intact astrocytes. Through monitoring the occupancy of the injured area, FAB7-KO astrocytes showed a slower proliferation rate compared with WT astrocytes after 48 hr, which was confirmed by BrdU immunostaining. There were no differences in cell migration and polarity of reactive astrocytes between FABP-KO and WT. Conclusively, our data suggest that FABP7 is important in the proliferation of reactive astrocytes in the context of CNS injury.