Determination of tetracycline using imprinted polymethacrylates along with fluorescent CdTe quantum dots on plastic substrates
Determination of tetracycline using imprinted polymethacrylates along with fluorescent CdTe quantum dots on plastic substrates
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DOI：
10.1007/s00604-017-2118-2
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发表时间：
2017-05-01
影响因子：
5.7
通讯作者：
Chen, Jian-Lian
中科院分区：
文献类型：
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作者：
Chen, Jian-Lian
以甲基丙烯酸、烯丙基甲基丙烯酸酯和二甲基丙烯酸乙二醇酯为原料，在柔性塑料聚酰亚胺片表面合成了由CdTe量子点和四环素印迹的聚甲基丙烯酸酯组成的分子印迹。优化了自由基引发聚合反应中CdTe水溶液的用量、乙醇稀释剂的用量、反应时间和反应温度。然后将MIP-QD复合材料与甲基丙烯酸酯化聚酰亚胺共聚。然后将所得膜浸入超声水-乙醇浴中以剥离四环素（Tc）模板。在薄膜上滴加Tc样品之前和之后，在315 nm激发下在565 nm处测量的荧光猝灭强度用于优化工艺。在pH 7.5下，校准图在70 μ M至2.2 mM Tc浓度范围内呈线性，LOD为8.8 μ M。相对标准偏差为8.2%（n = 10）。方法用于牛血清白蛋白和胎牛血清中Tc的测定，回收率为98%（200 μ g/ml）。mL（-1））和97%（1.0 ppt）。
Molecular imprints (MIPs) composed of CdTe quantum dots and tetracycline-imprinted polymethacrylates were synthesized on the surface of flexible plastic polyimide sheets from methacrylic acid, allyl mercaptan, and ethylene glycol dimethacrylate. The amount of aqueous CdTe solution, ethanol diluent, the reaction time and temperature of the radical-initiated polymerization were optimized. The MIP-QD composites were then copolymerized on the methacrylated polyimides. The resulting films were then immersed into a supersonic water-ethanol bath to strip off the tetracycline (Tc) templates. The fluorescence quenching intensity measured at 565 nm under 315 nm excitation before and after dropping a Tc sample on the film was used to optimize the processes. The calibration plot is linear in the 70 mu M to 2.2 mM Tc concentration range at pH 7.5, and the LOD is 8.8 mu M. The relative standard deviation is 8.2% (for n = 10). The method was applied to the determination of Tc in spiked samples of bovine serum albumin and fetal bovine serum and gave recoveries of 98% (at 200 mu gai...mL(-1)) and 97% (at 1.0 ppt), respectively.