The Chinese medicine, Jianpi Huayu Decoction, inhibits the epithelial mesenchymal transition via the regulation of the Smad3/Smad7 cascade
The Chinese medicine, Jianpi Huayu Decoction, inhibits the epithelial mesenchymal transition via the regulation of the Smad3/Smad7 cascade
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中药健脾化瘀汤通过调节Smad3/Smad7级联抑制上皮间质转化
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Zhong C
中科院分区：
文献类型：
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作者：
Zhong C
背景：肝细胞癌（HCC）是世界上最常见、侵袭性最强的恶性肿瘤之一。在中国，传统医学常用于治疗癌症。其中健脾化瘀汤是临床治疗多发性肿瘤的典型方药。然而，JHD的确切功能和目标目前尚不清楚。本研究的目的是评估JHD对HCC的疗效。方法与结果：用JHD药物血清对肝癌SMMC7221细胞进行剂量依赖性和时间依赖性处理。Real-time PCR （RT-PCR）、western-blot （WB）和免疫荧光显微镜显示，JHD使Smad7 mRNA和蛋白水平升高，p-Smad3蛋白水平降低。随后，E-cadherin的表达水平升高，N-cadherin和Vimentin的表达水平降低。转移和侵袭最终被抑制，这是由伤口愈合和transwell侵袭试验确定的。丹参酮IIA （Tan IIA）治疗JHD的结果与JHD相似，表明它很可能是JHD的主要功能药物单体。裸鼠体内实验也显示了JHD对上皮间充质转化（EMT）的抑制作用。结论：JHD是一种有效的肝癌治疗策略。
Background: Hepatocellular carcinoma (HCC) is one of the most common and aggressive malignant tumors in the world. In China, traditional medicine is commonly used in the treatment of cancer. Among these medicines, Jianpi Huayu Decoction (JHD) is a typical clinical prescription against multiple tumors. However, the exact func- tion and targets of JHD are currently unknown. The aim of this study is to assess the ef cacy of JHD against HCC. Methods and results: Hepatic carcinoma SMMC7221 cells were treated with JHD drug-serum in a dose- and time- dependent manner. Real-time PCR (RT-PCR), western-blot (WB), and immuno uorescence microscopy revealed that JHD increased both the mRNA and protein levels of Smad7 and decreased the protein level of p-Smad3. It subse- quently increased the E-cadherin expression level and decreased those of N-cadherin and Vimentin. Metastasis and invasion were eventually inhibited, as determined by the wound healing and transwell invasion assays. Treatment of Tanshinone IIA (Tan IIA) showed similar results as JHD, indicating that it is most likely the main functional drug monomer of JHD. The in vivo assay in nude mice also revealed the ef cacy of JHD to inhibit epithelial mesenchymal transition (EMT). Conclusion: JHD was shown to be an effective therapeutic strategy against HCC.