Variation in the HA antigenicity of A(H1N1)pdm09-related swine influenza viruses
Variation in the HA antigenicity of A(H1N1)pdm09-related swine influenza viruses
复制标题
甲型H1N1pdm09相关猪流感病毒HA抗原性的变化
DOI：
10.1099/jgv.0.001569
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发表时间：
2021
影响因子：
3.8
通讯作者：
Ozawa Makoto
中科院分区：
文献类型：
--
作者：
Khalil Ahmed Magdy;Yoshida Reiko;Masatani Tatsunori;Takada Ayato;Ozawa Makoto
自2009年流感大流行以来，病原体‘A(H1N1)pdm09病毒’一直在人类和猪群中传播。虽然血凝素(HA)基因片段的系统发育分析表明，与人A(H1N1)pdm09病毒相比，与A(H1N1)pdm09相关的猪流感病毒(SwIAvs)具有更广泛的遗传多样性，但目前尚不清楚这种遗传多样性是否反映了HA的抗原差异。为了评估A(H1N1)pdm09相关swIAV的HA基因多样性对HA抗原性的影响，我们对2013-2018年在日本分离的12株swIAV进行了鉴定。我们使用了雪貂抗血清和一组针对早期甲型H1N1流感pdm09分离株的抗HA鼠单抗(MAbbs)。用雪貂抗血清进行中和试验表明，12株swIAv中有5株的HA抗原性与早期的甲型H1N1流感病毒pdm09株有显著差异。根据swIAV毒株的不同，mAbs也表现出不同的中和模式。此外，在5个抗原性不同的swIAV中发现的HA第190位的单一氨基酸替换是导致swIAV Has抗原性差异的关键决定因素之一。在两个抗原性不同的swIAv中发现了HA分子第185位和276位氨基酸的两个潜在的N-糖基化位点。这些结果表明，与A(H1N1)pdm09相关的swIAV中HA的遗传多样性与其HA抗原变异有关。我们的发现强调了监测的必要性，以监测具有公共卫生重要性的swIAV抗原变种的出现。
Since the influenza pandemic in 2009, the causative agent ‘A(H1N1)pdm09 virus’, has been circulating in both human and swine populations. Although phylogenetic analyses of the haemagglutinin (HA) gene segment have revealed broader genetic diversity of A(H1N1)pdm09-related swine influenza A viruses (swIAVs) compared with human A(H1N1)pdm09 viruses, it remains unclear whether the genetic diversity reflects the antigenic differences in HA. To assess the impact of the diversity of the HA gene of A(H1N1)pdm09-related swIAVs on HA antigenicity, we characterized 12 swIAVs isolated in Japan from 2013 to 2018. We used a ferret antiserum and a panel of anti-HA mouse monoclonal antibodies (mAbs) raised against an early A(H1N1)pdm09 isolate. The neutralization assay with the ferret antiserum revealed that five of the 12 swIAVs were significantly different in their HA antigenicity from the early A(H1N1)pdm09 isolate. The mAbs also showed differential neutralization patterns depending on the swIAV strains. In addition, the single amino acid substitution at position 190 of HA, which was found in one of the five antigenically different swIAVs but not in human isolates, was shown to be one of the critical determinants for the antigenic difference of swIAV HAs. Two potential N-glycosylation sites at amino acid positions 185 and 276 of the HA molecule were identified in two antigenically different swIAVs. These results indicated that the genetic diversity of HA in the A(H1N1)pdm09-related swIAVs is associated with their HA antigenic variation. Our findings highlighted the need for surveillance to monitor the emergence of swIAV antigenic variants with public health importance.