First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella.
First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella.
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DOI：
10.3390/microorganisms10081609
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发表时间：
2022-08-09
期刊：
影响因子：
4.5
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中科院分区：
文献类型：
--
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巴尔通体属（根瘤菌目：巴尔通体科）包括兼性细胞内革兰氏阴性α-变形菌，其主要寄生于红细胞和内皮细胞，以及巨噬细胞、单核细胞和树突细胞。尽管巴尔通体可以感染世界范围内的许多哺乳动物和节肢动物，但在有袋动物中感染巴尔通体的报道很少。事实上，到目前为止，仅在澳大利亚和美利坚合众国的有袋动物和/或相关体外寄生虫中检测到此类物质。本研究的目的是分离和表征分子，形态学和表型巴尔通体感染自由生活的有袋类动物在巴西潘塔纳尔，南美洲最大的湿地采样。2018年12月捕获了2只有袋类动物，2019年2月捕获了6只有袋类动物，共采样了8种小型哺乳动物：5种（62.5%）大尾袋鼠和3种（37.5%）单尾鼠。将所有血液样品提交用于巴尔通体属的qPCR。基于nuoG基因，预富集液体培养和巧克力琼脂固体培养。巴尔通体（Bartonella sp.）macrurus和M.南极洲1株巴尔通体分离株来自T.使用基于Illumina NovaSeq和Nanopore测序平台的混合方法，选择显示与Bartonella vinsonii复合体和Bartonella machadoae密切相关的macrurus血液样品（菌株117 A）进行全基因组测序。该菌株基因组全长2.35Mbp，平均C + G含量为38.8%，编码2013个基因，质粒全长29 kb，平均C + G含量为34.5%。此外，该菌株与属于B的巴尔通体属物种的平均核苷酸同一性（ANI）为85%。vinsonii组和B组分别为91%和91%。machadoae。基于53种巴尔通体基因组共有的291个蛋白质编码基因的系统基因组学分析将该菌株定位于接近B的位置。machadoae。将该新种命名为哈氏巴尔通体Bartonella harrusi sp. nov.，其特征在于具有小的嗜二氧化碳、微需氧和需氧杆，没有皮利和鞭毛。总之，本工作描述了一个从哈氏巴尔通体分离的新种的生化、表型和基因组特征。巴西潘塔纳尔湿地的长尾龙血液样本最后，根据全基因组测序分析获得的ANI值，对巴尔通体属成员的分类学分类进行了综述。
The genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.
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影响因子：
14.9
作者：
Benson DA;Cavanaugh M;Clark K;Karsch-Mizrachi I;Ostell J;Pruitt KD;Sayers EW
通讯作者：
Sayers EW
影响因子：
9.4
作者：
Birkenheuer, AJ;Levy, MG;Breitschwerdt, EB
通讯作者：
Breitschwerdt, EB
影响因子：
2.2
作者：
Duncan, Ashlee W.;Maggi, Ricardo G.;Breitschwerdt, Edward B.
通讯作者：
Breitschwerdt, Edward B.
影响因子：
2.7
作者：
Chiaradia Furquim, Maria Eduarda;do Amaral, Renan;Andre, Marcos Rogerio
通讯作者：
Andre, Marcos Rogerio
影响因子：
1.7
作者：
Andre, Marcos Rogerio;Dumler, John Stephen;Machado, Rosangela Zacarias
通讯作者：
Machado, Rosangela Zacarias