MOLECULAR DISTINCTIONS AMONG CLINICAL ISOLATES OF MYCOPLASMA-PNEUMONIAE
MOLECULAR DISTINCTIONS AMONG CLINICAL ISOLATES OF MYCOPLASMA-PNEUMONIAE
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DOI：
10.1128/jcm.28.7.1538-1540.1990
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发表时间：
1990-07-01
影响因子：
9.4
通讯作者：
BASEMAN, JB
中科院分区：
文献类型：
--
作者：
SU, CJ;DALLO, SF;BASEMAN, JB
应用基因组DNA限制性内切酶指纹图谱和肺炎支原体细胞粘附素P1基因亚克隆的Southern杂交技术对肺炎支原体临床分离株进行鉴定。在对29株肺炎支原体个体检测的基础上，建立了两个不同的群。第一组在DNA经HindIII酶切后显示一条12千碱基的条带，由菌株M129-B16和其他三株于20世纪60年代在华盛顿州获得的菌株组成。剩下的肺炎支原体属于第2组，缺少12千碱基的条带，包括20世纪40年代、70年代和80年代的样本。这一类别还包括从关节炎患者的滑液中分离出的唯一肺炎支原体。
Restriction enzyme fingerprinting of genomic DNA and Southern blots probed with subclones of the Mycoplasma pneumoniae cytadhesin P1 gene were used to characterize clinical isolates of M. pneumoniae. On the basis of the examination of 29 individual M. pneumoniae isolates, two distinct groups were established. Group 1, which displayed a 12-kilobase band following DNA digestion with HindIII, consisted of strain M129-B16 and three others obtained in the state of Washington during the 1960s. The remaining M. pneumoniae strains belonged to group 2, which lacked the 12-kilobase band and included samples from the 1940s, 1970s, and 1980s. This category also included the only M. pneumoniae strain isolated from the synovial fluid of an arthritic patient.