ROLE FOR DYSTROPHIN-ASSOCIATED GLYCOPROTEINS AND UTROPHIN IN AGRIN-INDUCED ACHR CLUSTERING
ROLE FOR DYSTROPHIN-ASSOCIATED GLYCOPROTEINS AND UTROPHIN IN AGRIN-INDUCED ACHR CLUSTERING
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DOI：
10.1016/0092-8674(94)90051-5
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发表时间：
1994-06-03
期刊：
影响因子：
64.5
通讯作者：
SCHELLER, RH
中科院分区：
文献类型：
--
作者：
CAMPANELLI, JT;ROBERDS, SL;SCHELLER, RH
突触形成的特征是在突触前和突触后细胞之间的接触部位聚集分子。聚集蛋白，一种参与调节这一过程的蛋白质，引起乙酰胆碱受体（AChR）的聚集。在这里，我们的特点是在肌肉细胞表面的聚集蛋白结合位点，显示该网站对应于α-肌营养不良蛋白聚糖，并提出证据表明，α-肌营养不良蛋白聚糖是功能相关的聚集蛋白活性。此外，我们证明，α-肌营养不良蛋白聚糖和adhalin，肌营养不良蛋白相关的糖蛋白复合物的组成部分，以及utrophin，共定位与聚集蛋白诱导的乙酰胆碱受体簇。因此，聚集蛋白可以通过启动或稳定突触特异性膜细胞骨架而起作用，所述细胞骨架继而充当突触分子集中在其上的支架。
Synapse formation is characterized by the accumulation of molecules at the site of contact between pre- and postsynaptic cells. Agrin, a protein implicated in the regulation of this process, causes the clustering of acetylcholine receptors (AChRs). Here we characterize an agrin-binding site on the surface of muscle cells, show that this site corresponds to alpha-dystroglycan, and present evidence that alpha-dystroglycan is functionally related to agrin activity. Furthermore, we demonstrate that alpha-dystroglycan and adhalin, components of the dystrophin-associated glycoprotein complex, as well as utrophin, colocalize with agrin-induced AChR clusters. Thus, agrin may function by initiating or stabilizing a synapse-specific membrane cytoskeleton that in turn serves as a scaffold upon which synaptic molecules are concentrated.