Copper ion and G-quadruplex-mediated fluorescent sensor for highly selective detection of bleomycin in actual samples.
Copper ion and G-quadruplex-mediated fluorescent sensor for highly selective detection of bleomycin in actual samples.
复制标题
DOI：
10.1016/j.saa.2021.120572
复制
发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Lingfeng Qin;Bo Feng;Qi Luo;ZiHang Zeng;Peng Zhang;Xiaosheng Ye;Taiping Qing
中科院分区：
文献类型：
--
作者：
Lingfeng Qin;Bo Feng;Qi Luo;ZiHang Zeng;Peng Zhang;Xiaosheng Ye;Taiping Qing
博莱霉素（BLM）剂量不当易导致肺纤维化、肺毒性等一系列副作用。因此，检测实际样品中博莱霉素的含量，有助于充分发挥其疗效，降低其毒性。在此，我们构建了一个铜离子和G-四链体介导的无标记传感器来检测BLM。该策略主要依靠BLM与铜离子的螯合作用，使铜离子在螯合后失去对荧光染料N-甲基中卟啉（NMM）的猝灭能力。在G-四链体的辅助下，通过观察荧光的变化可以检测样品中BLM的含量。BLM浓度在0.1 nM-75 nM范围内线性关系良好，检出限为0.1 nM。该传感器不涉及任何Fe 2+的标记或添加，即使在10种不同抗生素和11种不同金属离子存在下，仍具有良好的监测效果，可成功应用于血清和废水中BLM的检测。因此，我们相信这项工作在抗生素监测和环境保护方面具有巨大的潜力。
Improper dosage of Bleomycin (BLM) can easily lead to a series of side effects such as pulmonary fibrosis and pulmonary toxicity. Therefore, detecting the content of BLM in actual sample is very helpful to make full use of its therapeutic efficacy and reduce its toxicity. Herein, we constructed a copper ion and G-quadruplex mediated label-free sensor to detect BLM. The strategy mainly relies on the chelation of BLM to copper ions, which makes the copper ions lose the quenching ability to the fluorescent dye N-methylmesoporphyrin (NMM) after chelation. With the assistance of the G-quadruplex, the BLM content in the sample can be detected by observing the change in fluorescence. A good linear relationship can be clearly observed within the BLM concentration range of 0.1 nM-75 nM, and the limit of detection was derived as 0.1 nM. This sensor did not involve any labeling or addition of Fe2+, even in the presence of 10 different antibiotics and 11 different metal ions, it still has a good monitoring effect, and can be successfully applied to the detection of BLM in serum and wastewater. Thus, we believe that this work hold great potential in antibiotic monitoring and environmental protection.