Comparative Study on the Differentiation of Mesenchymal Stem Cells Between Fetal and Postnatal Rat Spinal Cord Niche
Comparative Study on the Differentiation of Mesenchymal Stem Cells Between Fetal and Postnatal Rat Spinal Cord Niche
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胎儿与产后大鼠脊髓微环境间充质干细胞分化的比较研究
DOI：
10.3727/096368915x689910
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发表时间：
2016-01-01
影响因子：
3.3
通讯作者：
Yuan, Zhengwei
中科院分区：
文献类型：
--
作者：
Cao, Songying;Wei, Xiaowei;Yuan, Zhengwei
在之前的研究中，我们建立了一种产前手术方法，将间充质干细胞（MSCs）移植到胎鼠脊柱中来治疗神经管缺陷（NTDs）。我们发现移植的间充质干细胞存活并分化为神经系细胞。脊髓生态位中多种细胞因子和细胞外信号系统在细胞分化中起重要作用。本研究通过观察移植骨髓间充质干细胞在不同脊髓壁龛中的分化情况，进一步观察不同发育阶段脊髓中神经营养因子和生长因子的表达，探讨骨髓间充质干细胞在不同脊髓壁龛中的分化机制。结果表明，移植的MSCs表达神经前体细胞（nestin）、神经胶质细胞（GFAP）和神经元（β -微管蛋白）标记物。E16、P1和P21大鼠移植MSCs中GFP(+)/Inestin（+）双阳性细胞比例分别为18.31%、12.18%和5.06%。E16、P1、P21大鼠GFP(+)/GFAP（+）双阳性细胞比例分别为32.01%、15.35%、12.56%。E16、P1和P21中GFP(+)/ β -微管蛋白（+）双阳性细胞比例分别为11.76%、7.62%和4.88%。骨髓间充质干细胞在胚胎脊髓中的分化率明显高于出生后脊髓(p
In a previous study, we established a prenatal surgical approach and transplanted mesenchymal stem cells (MSCs) into the fetal rat spinal column to treat neural tube defects (NTDs). We found that the transplanted MSCs survived and differentiated into neural lineage cells. Various cytokines and extracellular signaling systems in the spinal cord niche play an important role in cell differentiation. In this study, we observed the differentiation of transplanted MSCs in different spinal cord niches and further observed the expression of neurotrophic factors and growth factors in the spinal cord at different developmental stages to explore the mechanism of MSC differentiation in different spinal cord niches. The results showed that transplanted MSCs expressed markers of neural precursor cells (nestin), neurogliocytes (GFAP), and neurons (beta-tubulin). The percentages of GFP(+)/Inestin(+) double-positive cells in transplanted MSCs in E16, P1, and P21 rats were 18.31%, 12.18%, and 5.06%, respectively. The percentages of GFP(+)/GFAP(+) double-positive cells in E16, P1, and P21 rats were 32.01%, 15.35%, and 12.56%, respectively. The percentages of GFP(+)/beta-tubulin(+) double-positive cells in E16, P1, and P21 were 11.76%, 7.62%, and 4.88%, respectively. The differentiation rates of MSCs in embryonic spinal cords were significantly higher than in postnatal spinal cords (p