Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes.
Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes.
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DOI：
10.1093/nar/gkn771
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发表时间：
2008-12
影响因子：
14.9
通讯作者：
Poirier, Guy G.
中科院分区：
文献类型：
--
作者：
Gagne, Jean-Philippe;Isabelle, Maxim;Lo, Ken Sin;Bourassa, Sylvie;Hendzel, Michael J.;Dawson, Valina L.;Dawson, Ted M.;Poirier, Guy G.
聚(ADP-核糖)(PADPr)是由聚(ADP-核糖)聚合酶(PAPS)催化NAD的ADP-核糖基部分聚合而成的聚合物。PADPr在细胞内的动态周转对许多细胞过程是必不可少的，包括细胞周期的进展、DNA修复和维持基因组完整性以及细胞凋亡。尽管对多聚ADP-核糖化反应调节生理条件的认识有了长足的进步，尽管pADPr可以在广泛的生物学过程中发挥中介作用，但到目前为止还很少有pADPr结合蛋白被鉴定出来。本研究通过对pADPr结合蛋白的电子精细预测和大规模的蛋白质组分析，建立了一套完整的pADPr相关蛋白图谱。这些pADPr相关蛋白在网络中的可视化和建模不仅反映了聚合(ADP-核糖基)在几个途径中的广泛参与，而且还可以识别蛋白质靶标，从而为揭示pADPr在正常生理条件下以及在暴露于遗传毒性刺激后的调控功能提供新的线索。
Poly(ADP-ribose) (pADPr) is a polymer assembled from the enzymatic polymerization of the ADP-ribosyl moiety of NAD by poly(ADP-ribose) polymerases (PARPs). The dynamic turnover of pADPr within the cell is essential for a number of cellular processes including progression through the cell cycle, DNA repair and the maintenance of genomic integrity, and apoptosis. In spite of the considerable advances in the knowledge of the physiological conditions modulated by poly(ADP-ribosyl)ation reactions, and notwithstanding the fact that pADPr can play a role of mediator in a wide spectrum of biological processes, few pADPr binding proteins have been identified so far. In this study, refined in silico prediction of pADPr binding proteins and large-scale mass spectrometry-based proteome analysis of pADPr binding proteins were used to establish a comprehensive repertoire of pADPr-associated proteins. Visualization and modeling of these pADPr-associated proteins in networks not only reflect the widespread involvement of poly(ADP-ribosyl)ation in several pathways but also identify protein targets that could shed new light on the regulatory functions of pADPr in normal physiological conditions as well as after exposure to genotoxic stimuli.
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Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
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Bateman, Alex
影响因子：
64.8
作者：
Ahel, Ivan;Ahel, Dragana;West, Stephen C.
通讯作者：
West, Stephen C.
影响因子：
4.8
作者：
Aguiar, RCT;Takeyama, K;Shipp, MA
通讯作者：
Shipp, MA
影响因子：
8
作者：
Cohausz, O.;Blenn, C.;Althaus, F. R.
通讯作者：
Althaus, F. R.
影响因子：
13.8
作者：
Combet, C;Blanchet, C;Deléage, G
通讯作者：
Deléage, G