First Report of Putative Streptococcus pneumoniae Serotype 6D among Nasopharyngeal Isolates from Fijian Children
First Report of Putative Streptococcus pneumoniae Serotype 6D among Nasopharyngeal Isolates from Fijian Children
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DOI：
10.1086/606118
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发表时间：
2009-11-01
影响因子：
6.4
通讯作者：
Gilbert, Gwendolyn L.
中科院分区：
文献类型：
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作者：
Jin, Ping;Kong, Fanrong;Gilbert, Gwendolyn L.
背景。提出了一种假定的肺炎链球菌血清型6D，它是由wciN（β）引入血清型6B引起的。我们研究了来自斐济儿童的98个独特的血清6组分离株，其中三分之二的儿童至少接种过1剂7价肺炎球菌结合疫苗，以及来自澳大利亚儿童的51个侵袭性分离株。我们使用了一种聚合酶链反应（PCR）系统，该系统针对wciN（β）和区分血清型6A和6B的单核苷酸多态性wcip584g （6A）和wciP584a （6B）。先前通过Quellung反应和wciP584g PCR鉴定为6A的22株澳大利亚分离株中有2株（9%）和64株斐济分离株中有24株（38%）含有wciN(β)，并被指定为6C；先前通过Quellung反应和wciP584a PCR鉴定为6B的34株斐济分离株中有14株（41%）含有wciN(β)，并被指定为新的推定血清型6D。分离血清型6D患儿未接种PCV-7的比例（2/14[14%]）明显低于分离血清型6B患儿（11/20 [55%]）（P < 0.05）。这是首次报告自然发生的血清6D型肺炎链球菌。
Background. A putative Streptococcus pneumoniae serotype, 6D, resulting from the introduction of wciN(beta) into serotype 6B has been proposed.Methods. We studied 98 unique serogroup 6 isolates from Fijian children, two-thirds of whom had received at least 1 dose of 7-valent pneumococcal conjugate vaccine, and 51 invasive isolates from Australian children. We used a polymerase chain reaction (PCR) system that targets both wciN(beta) and the single-nucleotide polymorphism that differentiates serotypes 6A and 6B-wciP584g (6A) and wciP584a (6B).Results. Two (9%) of 22 Australian isolates and 24 (38%) of 64 Fijian isolates previously identified as 6A by the Quellung reaction and wciP584g PCR contained wciN(beta) and were designated as 6C; 14 (41%) of 34 Fijian isolates previously identified as 6B by the Quellung reaction and wciP584a PCR contained wciN(beta) and were designated as the new putative serotype 6D. A significantly smaller proportion of children from whom serotype 6D was isolated (2/14 [14%]) had not received PCV-7, compared with the proportion of those from whom serotype 6B was isolated (11/20 [55%]) (P < .05).Conclusion. This is the first report of naturally occurring S. pneumoniae serotype 6D.