SIGNAL-TRANSDUCTION OF A G-PROTEIN-COUPLED RECEPTOR IN CAVEOLAE - COLOCALIZATION OF ENDOTHELIN AND ITS RECEPTOR WITH CAVEOLIN
SIGNAL-TRANSDUCTION OF A G-PROTEIN-COUPLED RECEPTOR IN CAVEOLAE - COLOCALIZATION OF ENDOTHELIN AND ITS RECEPTOR WITH CAVEOLIN
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DOI：
10.1073/pnas.91.24.11728
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发表时间：
1994-11-22
影响因子：
11.1
通讯作者：
LODISH, HF
中科院分区：
文献类型：
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作者：
CHUN, MY;LIYANAGE, UK;LODISH, HF
小窝是质膜直径为50-100 nm的小内陷。由于钙通道、肌醇1，4，5-三磷酸受体和异源三聚体GTP结合蛋白（G蛋白）位于小窝中，它们可能参与G蛋白偶联受体的信号转导。在这里，我们表明，G蛋白偶联内皮素受体亚型A（ET（A））和它的结合内皮素配体被发现在质膜小窝。在表达转染ET（A）受体的小鼠的提取物中，ET（A）及其结合配体与小窝蛋白（小窝的结构成分）共免疫沉淀。共聚焦荧光显微镜显示在没有内皮素配体的情况下，内皮素（A）受体和小窝蛋白在质膜上或质膜附近的微斑中共定位。这些观察结果表明质膜小窝在信号转导中的功能作用，这G蛋白偶联受体。
Caveolae are small invaginations of the plasma membrane 50-100 nm in diameter. Since calcium channels, inositol 1,4,5-trisphosphate receptors, and heterotrimeric GTP-binding proteins (G proteins) are localized in caveolae, they may participate in signal transduction by G protein-coupled receptors. Here we show that the G protein-coupled endothelin receptor subtype A (ET(A)) and its bound endothelin ligand are found in plasma membrane caveolae. ET(A) and its bound ligand coimmunoprecipitate with caveolin, a structural component of caveolae, in extracts of tells expressing transfected ET(A) receptors. Confocal fluorescence microscopy shows colocalization of ET(A) receptors and caveolin in micropatches at or near the plasma membrane, in the absence of endothelin ligands. These observations demonstrate a functional role for plasma membrane caveolae in signal transduction by this G protein-coupled receptor.