A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen
A graphene oxide-peptide fluorescence sensor for proteolytically active prostate-specific antigen
复制标题
用于蛋白水解活性前列腺特异性抗原的氧化石墨烯肽荧光传感器
DOI：
10.1039/c2mb05379a
复制
发表时间：
2012-01-01
影响因子：
--
通讯作者：
Ma, Huimin
中科院分区：
文献类型：
--
作者：
Feng, Tingting;Feng, Duan;Ma, Huimin
本文提出了一种新的传感器，检测蛋白水解活性的前列腺特异性抗原（PSA）通过组装一个专门设计的FITC标记的肽与氧化石墨烯（GO）。染料标记的肽的荧光在GO的存在下淬灭。传感器与PSA的反应切割肽，导致染料部分的释放以及以剂量和时间依赖性方式的荧光强度的大幅增加，并且可以相应地定量PSA。与现有方法相比，该方法简单，因为基于GO-肽的传感器易于组装，并且可以在不涉及复杂程序的情况下实现检测。此外，该方法的适用性已被证明是通过检测PSA在加标尿样。
This paper presents a novel sensor to detect proteolytically active prostate-specific antigen (PSA) by assembling a purpose-designed FITC-labeled peptide with graphene oxide (GO). The fluorescence of the dye-labeled peptide was quenched in the presence of GO. Reaction of the sensor with PSA cleaves the peptide, leading to the release of the dye moiety and a great increase in fluorescence intensity in a dose- and time-dependent manner, and PSA can be quantified accordingly. This approach is simple compared to existing methods since the GO-peptide-based sensor is easily assembled and detection can be achieved without the involvement of complicated procedures. Moreover, the applicability of the method has been demonstrated by detecting PSA in spiked urine samples.