DIFFERENTIATION OF VASCULAR ENDOTHELIUM IN CHICK CHORIOALLANTOIS - STRUCTURAL AND AUTORADIOGRAPHIC STUDY
DIFFERENTIATION OF VASCULAR ENDOTHELIUM IN CHICK CHORIOALLANTOIS - STRUCTURAL AND AUTORADIOGRAPHIC STUDY
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DOI：
10.1016/0012-1606(74)90004-9
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发表时间：
1974-01-01
影响因子：
2.7
通讯作者：
FOLKMAN, J
中科院分区：
文献类型：
--
作者：
AUSPRUNK, DH;KNIGHTON, DR;FOLKMAN, J
用光镜和电镜观察了受精后8 ~ 18天鸡胚绒毛尿囊膜血管内皮细胞的成熟过程。光显微镜放射自显影管理后的氚化胸苷被用来确定在不同的发展阶段的内皮细胞群的胸苷标记指数。结果表明，在孵育第11天之前，内皮细胞具有未成熟和相对未分化细胞的形态特征。在此期间，它们表现出约23%的高标记指数。在第11天，标记指数降低至2.8%，随后细胞开始获得成熟分化内皮的结构特征。内皮细胞标记的模式表明，在高内皮细胞有丝分裂期间，生长中的绒毛尿囊膜的毛细血管网络通过现有毛细血管中内皮细胞的整体增殖而扩展，而不是通过形成新的毛细血管芽。年轻的绒毛膜尿囊中内皮细胞的不成熟，或者相反地，它们的高细胞分裂率，可能会影响该膜在第11天之前支持移植组织的能力。
The maturation of vascular endothelial cells in the chick chorioallantoic membrane, from 8 to 18 days after fertilization, was investigated by light and electron microscopy. Light microscopic autoradiography following administration of tritiated thymidine was used to determine the thymidine labeling index of the endothelial cell population at various stages of development. Results indicate that, prior to day 11 of incubation, endothelial cells have the morphological characteristics of immature and relatively undifferentiated cells. During this time they exhibit a high labeling index of approximately 23%. At 11 days, the labeling index decreases to 2.8%, and subsequently the cells begin to acquire structural characteristics of mature differentiated endothelium. The pattern of endothelial cell labeling suggests that, during the period of high endothelial cell mitosis, the capillary network of the growing chorioallantoic membrane is expanding by an overall proliferation of endothelial cells in existing capillaries, rather than by formation of new capillary sprouts. The immaturity of endothelial cells in the young chorioallantois, or conversely their high rate of cell division, may influence the ability of this membrane to support grafted tissue prior to day 11.