DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis
DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis
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DOI：
10.1104/pp.105.061689
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发表时间：
2005-06-01
期刊：
影响因子：
7.4
通讯作者：
Fukuda, H
中科院分区：
文献类型：
--
作者：
Sawa, S;Koizumi, K;Fukuda, H
在大多数双子叶植物中，叶片中的维管组织具有网状脉序。我们分离并鉴定了一个维管网缺陷突变体van3。VAN3突变体表现出不连续的血管模式，而VAN3编码一种ADP-核糖化因子-GTP酶激活蛋白，调节跨高尔基体网络中的膜运输。为了阐明通过膜转运控制静脉构型过程的分子本质，我们使用酵母(Saccharmyces Cerevisiae)双杂交系统寻找与VAN3相互作用的蛋白质。因此，我们分离了植物动力素相关蛋白1A(DRP1A)作为VAN3相互作用蛋白。DRP1A：：GUS和VAN3：：GUS的时空表达模式非常相似。VAN3和DRP1A的亚细胞定位完全重叠。Drp1a表现为不连通的血管网络，并且drp1a突变增强了drp1a van3双突变体的血管不连续表型。此外，Drp1突变增强了gnom突变体的不连续血管模式，其效果与van3突变相同。这些结果表明，Drp1调控VAN3在囊泡萌发过程中的功能，而VAN3是通过跨高尔基网络调节拟南芥维管形成的。
In most dicotyledonous plants, vascular tissues in the leaf have a reticulate venation pattern. We have isolated and characterized an Arabidopsis ( Arabidopsis thaliana) mutant defective in the vascular network defective mutant, van3. van3 mutants show a discontinuous vascular pattern, and VAN3 is known to encode an ADP- ribosylation- factor- GTPase- activating protein that regulates membrane trafficking in the trans- Golgi network. To elucidate the molecular nature controlling the vein patterning process through membrane trafficking, we searched VAN3- interacting proteins using a yeast ( Saccharomyces cerevisiae) two hybrid system. As a result, we isolated the plant Dynamin- Related Protein 1A ( DRP1A) as a VAN3 interacting protein. The spatial and temporal expression patterns of DRP1A::GUS and VAN3::GUS were very similar. The subcellular localization of VAN3 completely overlapped to that of DRP1A. drp1a showed a disconnected vascular network, and the drp1a mutation enhanced the phenotype of vascular discontinuity of the van3 mutant in the drp1a van3 double mutant. Furthermore, the drp1 mutation enhanced the discontinuous vascular pattern of the gnom mutant, which had the same effect as that of the van3 mutation. These results indicate that DRP1 modulates the VAN3 function in vesicle budding from the trans- Golgi network, which regulates vascular formation in Arabidopsis.