Nanogel formation consisting of DNA and poly(amido amine) dendrimer studied by static light scattering and atomic force microscopy.
Nanogel formation consisting of DNA and poly(amido amine) dendrimer studied by static light scattering and atomic force microscopy.
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DOI：
10.1021/bm034239u
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发表时间：
2004
影响因子：
6.2
通讯作者：
A. Mitra;T. Imae
中科院分区：
文献类型：
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作者：
A. Mitra;T. Imae
结合的聚（酰胺基胺）树枝状大分子与鲑鱼睾丸DNA在0.01 M NaCl的水溶液中，在pH 6.5进行了研究。静态光散射、紫外光谱和圆二色光谱的物理化学实验表明，当树枝状聚合物/DNA的混合比（树枝状聚合物中NH_2基团与DNA中磷酸基团的数量比）为0.2时，由于树枝状聚合物与DNA链的结合，DNA的构象发生了显著的变化。当混合比增加到0.2以上时，树枝状聚合物/DNA复合物形成聚集体（纳米凝胶）。重均分子量，回转半径，和浊度的测量结果表明，聚集体的大小增加到0.8的混合比。原子力显微镜图像证实了复合物的形成和纳米凝胶的形态。
Binding of a poly(amido amine) dendrimer with salmon testes DNA in an aqueous solution of 0.01 M NaCl at pH 6.5 has been investigated. It was shown from the physicochemical experiments of static light scattering and ultra-violet and circular dichroism spectroscopy that at a 0.2 mixing ratio of dendrimer/DNA (number ratio of NH2 groups in dendrimer vs phosphate groups in DNA) significant conformational change of the DNA occurred owing to the binding of dendrimers on the DNA chain. The dendrimer/DNA complexes formed aggregates (nanogels) when the mixing ratio was increased above 0.2. Weight-averaged molecular weight, radius of gyration, and turbidity measurements revealed that the size of the aggregates increased up to a mixing ratio of 0.8. Atomic force microscopic images certified the formation of complexes and the morphology of the nanogels.