Peripheral Microvascular Vasodilatory Response to Estradiol and Genistein in Women with Insulin Resistance.
Peripheral Microvascular Vasodilatory Response to Estradiol and Genistein in Women with Insulin Resistance.
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DOI：
10.1111/micc.12208
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发表时间：
2015-07
期刊：
影响因子：
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通讯作者：
Stachenfeld NS
中科院分区：
文献类型：
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作者：
Wenner MM;Taylor HS;Stachenfeld NS
雌激素增强健康女性的血管舒张，但植物雌激素染料木黄酮的血管效应仍在研究中。胰岛素抵抗（IR）损害微血管功能。因此，我们研究了雌二醇，金雀异黄素和IR对微血管舒张反应的相互作用。我们假设雌二醇和染料木黄酮会增加健康女性（对照组，n=8，23±2岁，BMI 25.9±2.9 kg/m2）的微血管舒张，但不会增加IR女性（n=7，20±1岁，BMI 27.3±3.0 kg/m2）的微血管舒张。我们使用皮肤循环作为微血管舒张功能的模型。我们在局部皮肤加热（42°C）期间用雌二醇或金雀异黄素微透析灌注，用激光多普勒血流仪和逐搏血压测定皮肤血管传导性（CVC）。由于热诱导的血管舒张主要是一个NO介导的反应，我们研究了微血管舒张与L-NMMA。在对照组妇女中，雌二醇增强CVC（94.4± 2. 6% vs.生理盐水81.6± 4.2%CVCmax，P<0.05），L-NMMA逆转CVC（80.9± 7.8%CVCmax，P<0.05），但染料木素不影响血管舒张。雌二醇和染料木黄酮都不改变IR中的CVC，尽管L-NMMA在染料木黄酮期间减弱CVC。我们的研究不支持改善健康年轻女性在金雀异黄素暴露期间的微血管反应性，并表明雌二醇和金雀异黄素都不能改善IR女性的微血管舒张反应性。
Estradiol enhances vasodilation in healthy women, but vascular effects of the phytoestrogen genistein are still under investigation. Insulin resistance (IR) compromises microvascular function. We therefore examined the interaction of estradiol, genistein, and IR on microvascular vasodilatory responsiveness. We hypothesized that estradiol and genistein increase microvascular vasodilation in healthy women (control, n=8, 23±2 yr, BMI 25.9±2.9 kg/m2) but not in women with IR (n=7, 20±1 yr, BMI 27.3±3.0 kg/m2). We used the cutaneous circulation as a model of microvascular vasodilatory function. We determined cutaneous vascular conductance (CVC) with laser Doppler flowmetry and beat-to-beat blood pressure during local cutaneous heating (42°C) with estradiol or genistein microdialysis perfusions. Because heat induced vasodilation is primarily an NO mediated response, we examined microvascular vasodilation with and without L-NMMA. In control women, estradiol enhanced CVC (94.4±2.6 % vs. saline 81.6±4.2 % CVCmax, P<0.05), which was reversed with L-NMMA (80.9±7.8 % CVCmax, P<0.05), but genistein did not affect vasodilation. Neither estradiol nor genistein altered CVC in IR, although L-NMMA attenuated CVC during genistein. Our study does not support improved microvascular responsiveness during genistein exposure in healthy young women, and demonstrates that neither estradiol nor genistein improve microvascular vasodilatory responsiveness in women with IR.