Dual functional nisin-multi-walled carbon nanotubes coated filters for bacterial capture and inactivation.
Dual functional nisin-multi-walled carbon nanotubes coated filters for bacterial capture and inactivation.
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DOI：
10.1186/s13036-015-0018-8
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发表时间：
2015
影响因子：
5.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Dong X;Yang L
从水中去除病原体是预防水传播疾病的一种方法。本论文利用多壁碳纳米管（MWCNTs）包覆在市售滤膜上，并利用其优异的吸附性能吸附天然抗菌肽-乳酸链球菌素（Nisin），研制了两种不同外层直径的MWCNTs改性滤膜（MWCNT 1：直径<8 nm; MWCNT 2：直径10-20 nm）。MWCNT层的厚度，表面形态，和两种类型的MWCNT涂层过滤器的表面疏水性进行了表征。过滤器上的MWCNT涂层显著增加了表面疏水性。乳酸链球菌素的吸收和细菌病原体的捕获与增加的表面疏水性相关。具有1.5 mg MWCNT负载的MWCNTs 1和MWCNTs 2过滤器从总共含有约106 CFU/mL细胞的水溶液中分别捕获2.44和3.88 log细胞。多壁碳纳米管过滤器表面上的0.5 mg量的乳链菌肽存款显著降低了捕获的B的生存能力。对炭疽菌的抑制率为95.71- 97.19%，对炭疽菌代谢活性的抑制率约为98.3%。结果表明，MWCNT-nisin过滤器在一个单一的过滤步骤中实现了细菌病原体捕获和抑制的双重功能，这可能适用于从水源中去除不期望的微生物和抑制捕获的革兰氏阳性菌活性。
Removal of pathogens from water is one way to prevent waterborne illness. In this paper, we developed dual functional carbon nanotube (CNT) modified filters for bacterial capture and inactivation, utilizing multi-walled CNTs (MWCNTs) to coat on commercially available filters and making use of the exceptional adsorption property of CNTs to adsorb a natural antimicrobial peptide-nisin on it. Two types of MWCNTs with different outer layer diameters were used (MWCNTs1: <8 nm in diameter; MWCNTs2: 10–20 nm in diameter). The thickness of MWCNT layers, surface morphology, and surface hydrophobicity of both types of MWCNT coated filters were characterized. The MWCNT coating on filters significantly increased the surface hydrophobicity. The absorption of nisin and the capture of bacterial pathogens were correlated with increased surface hydrophobicity. The MWCNTs1 and MWCNTs2 filters with 1.5 mg MWCNTs loading captured 2.44 and 3.88 log of cells, respectively, from aqueous solutions containing a total of ~106 CFU/mL cells. Nisin deposit at the amount of 0.5 mg on the surfaces of MWCNT filters significantly reduced the viability of captured B. anthracis cells by 95.71–97.19 %, and inhibited the metabolic activities of the captured cells by approximately 98.3 %. The results demonstrated that the MWCNT-nisin filters achieved dual functions in bacterial pathogen capture and inhibition in one single filtration step, which is potentially applicable in removing undesired microorganisms from water sources and inhibiting captured Gram positive bacteria activities.