Food capture and adhesion by the heliozoonActinophrys sol
Food capture and adhesion by the heliozoonActinophrys sol
复制标题
日光虫Actinophrys sol 的食物捕获和粘附
作者：
M. Sakaguchi;K. Hausmann;T. Suzaki
太阳虫Actinophrys sol的特征是从球形细胞体中放射出针状轴足。当日光虫捕获食物生物时，猎物通过粘附在轴足表面而被捕获，轴足表面靠近质膜处存在许多外泌体。为了了解日虫捕获猎物生物的分子机制，进行了粘附物质的粗分离和表征。被捕食鞭毛虫（长杆绿原藻）粘附并聚集在通过冷冻或高温处理（80 °C，10分钟）杀死的日虫体细胞的残余物上。分离的外泌体，这是制备的细胞匀浆和离心后，冷冻和解冻的上清液，表现出强烈的粘附的猎物鞭毛虫响应的上清液粘附其鞭毛和细胞体彼此形成花束状的细胞簇。从热处理的A中进一步提取粘合剂物质。Sol.这部分含有丝状物质的分泌内容物的extrusomes在喂养过程中观察到的。胰蛋白酶处理不抑制其粘附活性。
SummaryThe heliozoonActinophrys sol is characterized by needle-like axopodia radiating from the spherical cell body. When helio-zoons capture food organisms, the prey is caught by adhesion to the surface of axopodia where numerous extrusomes are present close to the plasma membrane. To understand the molecular mechanism by which the heliozoons capture prey organisms, crude isolation and characterization of the adhesive substance was carried out. Prey flagellates (Chlorogonium elongatum) adhered and aggregated to remnants of heliozoon cells which had been killed by freezing or treatment at high temperature (80 °C for 10 min). Isolated extrusomes, which were prepared as the supernatant of cells homogenized and centrifuged after freezing and thawing, showed strong adhesion to the prey flagellates which responded to the supernatant by adhering their flagella and cell bodies to each other to form bouquet-like cell clusters. The adhesive substance was further extracted from heat-treatedA. sol. This fraction contained filamentous material similar to the secreted contents of the extrusomes observed during feeding. Its adhesive activity was not inhibited by trypsin treatment.