GENERATION FROM ARACHIDONIC ACID OF RABBIT AORTA CONTRACTING SUBSTANCE (RCS) BY A MICROSOMAL ENZYME PREPARATION WHICH ALSO GENERATES PROSTAGLANDINS
GENERATION FROM ARACHIDONIC ACID OF RABBIT AORTA CONTRACTING SUBSTANCE (RCS) BY A MICROSOMAL ENZYME PREPARATION WHICH ALSO GENERATES PROSTAGLANDINS
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DOI：
10.1111/j.1476-5381.1972.tb08142.x
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发表时间：
1972-01-01
影响因子：
7.3
通讯作者：
VANE, JR
中科院分区：
文献类型：
--
作者：
GRYGLEWSKI, R;VANE, JR
1. 将花生四烯酸钠与由狗脾制成的粗制前列腺素合成酶制剂一起孵育。在动态系统中进行孵育，以便将产物递送至兔主动脉和大鼠胃的条带。 2.形成兔主动脉收缩物质(RCS)和前列腺素样物质。 RCS 与过敏反应中释放的 RCS 具有相似的特性。它不稳定，随着 RCS 活性下降，前列腺素样活性增加。前列腺素合成酶抑制剂（例如吲哚美辛）可以阻止其形成。 3.通过将花生四烯酸与脂氧合酶一起温育，形成一种不同的兔主动脉收缩物质，脂氧合酶产生过氧化物。这种物质很稳定，不会导致前列腺素的产生。 4. 我们的结论是，RCS 可能是前列腺素生成的中间体，例如假设的环内过氧化物。
1. Sodium arachidonate was incubated with a crude prostaglandin synthetase preparation made from dog spleen. The incubation was made in a dynamic system so that the products could be delivered to strips of rabbit aorta and rat stomach. 2. A rabbit aorta contracting substance (RCS) and a prostaglandin-like substance were formed. The RCS had similar properties to that released in anaphylaxis. It was unstable and as the RCS activity declined, so the prostaglandin-like activity increased. Its formation was prevented by prostaglandin synthetase inhibitors such as indomethacin. 3. A different rabbit aorta contracting substance was formed by incubation of arachidonate with lipoxygenase, which generates peroxides. This substance was stable and did not lead to prostaglandin production. 4. We conclude that RCS may be an intermediate in prostaglandin production, such as the postulated cyclic endoperoxide.