Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam
Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam
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DOI：
10.1111/j.1472-765x.2012.03287.x
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发表时间：
2012-10-01
影响因子：
2.4
通讯作者：
Vandamme, P.
中科院分区：
文献类型：
--
作者：
Doan, N. T. L.;Van Hoorde, K.;Vandamme, P.
目的：评估MALDI-TOF MS在快速可靠地分类和鉴定传统发酵食品中乳酸菌（LAB）方面的潜在用途。方法与结果：从发酵肉制品中分离出119株乳酸菌聚类分析结果表明，在（GTG）5-PCR和MALDI-TOF MS两种方法中，有5个种被同一个分离物所代表，在（GTG）5-PCR和MALDI-TOF MS两种方法中，有5个种被归为一类;然而，可以观察到所描绘的（GTG）5-PCR和MALDI-TOF MS簇之间的最小相似性的差异：与（GTG）5-PCR谱相比，三个物种的MALDI-TOF MS谱显示出更多的异质性;在相应的（GTG）5-PCR树状图中细分了两个物种，每个物种由单个MALDI-TOF簇表示。作为MALDI-TOF MS鉴定潜力的证明，使用MALDI-TOF MS分析来自一个发酵芥末样品的LAB多样性。使用PheS基因测序进行验证。结论：MALDI-TOF MS是一种功能强大、快速、可靠且具有成本效益的技术，可用于鉴定与发酵食品生产相关的LAB。研究的意义和影响：可以使用MALDI-TOF MS鉴定食品LAB，其应用可能扩展到其他食品基质和/或其他食品来源的微生物。
Aims: To evaluate the potential use of MALDI-TOF MS for fast and reliable classification and identification of lactic acid bacteria (LAB) from traditional fermented foods. Methods and Results: A total of 119 strains of LAB from fermented meat (nem chua) were analysed with both (GTG)5-PCR fingerprinting and MALDI-TOF MS. Cluster analysis of the profiles revealed five species represented by a single isolate both in (GTG)5-PCR and in MALDI-TOF MS; five species grouped alike for (GTG)5-PCR and for MALDI-TOF MS; however, differences in minimal similarity between the delineated (GTG)5-PCR and MALDI-TOF MS clusters could be observed; three species showed more heterogeneity in their MALDI-TOF MS profiles compared to their (GTG)5-PCR profiles; two species, each represented by a single MALDI-TOF cluster, were subdivided in the corresponding (GTG)5-PCR dendrogram. As proof of the identification potential of MALDI-TOF MS, LAB diversity from one fermented mustard sample was analysed using MALDI- TOF MS. PheS gene sequencing was used for validation. Conclusions: MALDI-TOF MS is a powerful, fast, reliable and cost-effective technique for the identification of LAB associated with the production of fermented foods. Significance and Impact of the Study: Food LAB can be identified using MALDI-TOF MS, and its application could possibly be extended to other food matrices and/or other food-derived micro-organisms.