Combined CatWalk Index: an improved method to measure mouse motor function using the automated gait analysis system.
Combined CatWalk Index: an improved method to measure mouse motor function using the automated gait analysis system.
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DOI：
10.1186/s13104-018-3374-x
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发表时间：
2018-04-27
影响因子：
1.8
通讯作者：
Itaka K
中科院分区：
文献类型：
--
作者：
Crowley ST;Kataoka K;Itaka K
测量小鼠的运动功能对于研究脊髓损伤(SCI)或其他疾病的模型很重要。有几种方法是基于对老鼠在开阔地带移动的目测。虽然这些方法只需要很少的设备，但观察员必须接受培训，人为错误或主观性的可能性是无法消除的。Noldus T型台XT自动步态分析系统通过拍摄老鼠的高分辨率视频来评估老鼠的运动功能，并使用专门的软件来测量动物步态的几个方面。这种仪器减少了人为错误的可能性，但并不总是清楚哪些数据对于评估运动功能是重要的。这项研究使用了在老鼠SCI实验中收集的数据来创建一个简单的数学模型，该模型将T型台系统收集的数据组合成一个单一的分数，即组合T型台指数或CCI。CCI系统产生的结果与Basso Mouse Scale或T型台的步序规则指数相似。然而，CCI的变异系数比其他两种方法都要小得多。此外，CCI评分与冲击力的相关性略好一些。CCI系统很可能成为国际SCI研究的有用工具。本文的在线版本(10.1186/s13104-0183374-x)包含补充材料，授权用户可以使用。
Measuring motor function in mice is important for studying models of spinal cord injury (SCI) or other diseases. Several methods exist based on visual observation of mice moving in an open field. Though these methods require very little equipment, observers must be trained, and the possibility of human error or subjectivity cannot be eliminated. The Noldus CatWalk XT Automated Gait Analysis system assesses mouse motor function by taking high-resolution videos of the mice, with specialized software to measure several aspects of the animal’s gait. This instrument reduces the possibility of human error, but it is not always clear what data is important for assessing motor function. This study used data collected during mouse SCI experiments to create a simple mathematical model that combines the data collected by the CatWalk system into a single score, the Combined CatWalk Index or CCI. The CCI system produces similar results to the Basso Mouse Scale or the CatWalk’s Step Sequence Regularity Index. However, the CCI has a significantly smaller coefficient of variation than either other method. Additionally, CCI scoring shows slightly better correlation with impact force. The CCI system is likely to be a useful tool for SCI research. The online version of this article (10.1186/s13104-018-3374-x) contains supplementary material, which is available to authorized users.