Metabolism of cyclopenta(cd)pyrene at the K-region by microsomes and a reconstituted cytochrome P-450 system from rat liver.
Metabolism of cyclopenta(cd)pyrene at the K-region by microsomes and a reconstituted cytochrome P-450 system from rat liver.
复制标题
微粒体和来自大鼠肝脏的重组细胞色素 P-450 系统在 K 区对环戊 (cd) 芘的代谢。
DOI：
10.1016/0006-291x(81)91917-3
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
Gold,A
中科院分区：
文献类型：
--
作者：
Eisenstadt,E;Shpizner,B;Gold,A
从苯巴比妥或3-甲基胆蒽处理的大鼠肝微粒体和重组细胞色素P-450系统将其K区的环戊烯(CD)代谢成反式-9，10-二羟基-9，10-二氢环戊烯(CD)。K区产物的生成率为反式-3，4-二羟基-3，4-二氢环戊二烯(CD)的5%~25%。微粒体和纯化的细胞色素P-450优先选择乙烯C(3)-C(4)位上的环戊二烯(CD)芘，部分原因是C(4)位在最高占据的分子轨道上具有最高的电子密度。
Liver microsomes and reconstituted cytochrome P-450 systems purified from phenobarbital or 3-methylcholanthrene pre-treated rats metabolize cyclopenta (cd) pyrene at its K-region to trans-9, 10-dihydroxy-9, 10-dihydrocyclopenta (cd) pyrene. The rate of formation of the K-region product is from 5% to 25% that of trans-3, 4-dihydroxy-3, 4-dihydro-cyclopenta (cd) pyrene. The preference of microsomes and purified cytochromes P-450 for oxygenating cyclopenta (cd) pyrene at the ethylenic C (3)–C (4) position is explainable in part by the fact that the C (4) position has the greatest electron density in the highest occupied molecular orbital.