Novel peptide neuronal system in rat brain and pituitary.
Novel peptide neuronal system in rat brain and pituitary.
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大鼠大脑和垂体中的新型肽神经元系统。
DOI：
10.1126/science.7029714
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Barchas,JD
中科院分区：
文献类型：
--
作者：
Weber,E;Evans,CJ;Samuelsson,SJ;Barchas,JD
用软体动物心兴奋肽（软体动物心兴奋肽）抗体对大鼠中枢神经系统进行免疫组织荧光研究，发现在大脑、脊髓和垂体后叶中广泛存在神经元系统。免疫反应性轴突和细胞体主要位于皮层、边缘和下丘脑区。脑和垂体连续切片的免疫染色显示，Phe-Met-Arg-Phe-NH2免疫反应神经元与Met-enkephalin或假设的Met-enkephalin前体Tyr-Gly-Gly-Phe-Met-Arg-Phe - nh2抗体标记的神经元不同，后者在结构上与Phe-Met-Arg-Phe-NH2相关。抗血清吸收对照染色和放射免疫分析表明，引起特异性免疫荧光识别的肽在羧基端具有修饰的Arg-Phe序列。
Immunohistofluorescence studies of the rat central nervous system with antibodies to Phe-Met-Arg-Phe-NH2(molluskan cardioexcitatory peptide) revealed a widespread neuronal system in the brain, spinal cord, and posterior pituitary. Immunoreactive axons and cell bodies were mainly located in cortical, limbic, and hypothalamic areas. Immunostaining of serial sections of the brain and pituitary showed that the Phe-Met-Arg-Phe-NH2 immunoreactive neurons were different from neurons labeled by antibodies to either Met-enkephalin or the putative Met-enkephalin precursor Tyr-Gly-Gly-Phe-Met-Arg-Phe, which is structurally related to Phe-Met-Arg-Phe-NH2. Control staining by antiserum absorption and radioimmunoassay indicated that the antibodies that caused the specific immunofluorescence recognized peptides with an amidated Arg-Phe sequence at the carboxyl terminus.