The candidate sour taste receptor, PKD2L1, is expressed by type III taste cells in the mouse
The candidate sour taste receptor, PKD2L1, is expressed by type III taste cells in the mouse
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DOI：
10.1093/chemse/bjm083
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发表时间：
2008-03-01
期刊：
影响因子：
3.5
通讯作者：
Finger, Thomas E.
中科院分区：
文献类型：
--
作者：
Kataoka, Shinji;Yang, Ruibiao;Finger, Thomas E.
基于分子生物学和功能研究，瞬时受体电位通道PKD2L1被报道为酸味的候选受体。在这里，我们研究了PKD2L1免疫反应(IR)在小鼠味蕾中的表达模式。正如先前报道的那样，PKD2L1-IR存在于每个味蕾中的几个细长细胞中。表达PKD2L1的细胞不同于表达II型细胞标志物PLCβ2的细胞。同样，PKD2L1免疫反应阳性的味觉细胞不表达标记I型细胞的外源性ATPase。PKD2L1阳性细胞对神经细胞黏附分子、5-羟色胺、PGP-9.5(泛素羧基末端转移酶)和嗜铬粒蛋白A呈免疫反应，所有这些都存在于III型味觉细胞中。在超微结构水平上，PKD2L1免疫反应细胞与传入神经纤维形成突触，这是III型味觉细胞的另一个特征。这些结果与每个味蕾中不同的味觉细胞执行不同功能的观点一致。我们认为，III型细胞是传递和/或传递有关“酸”的信息所必需的，但在传递其他味道的味道信息方面几乎没有或几乎没有作用。
The transient receptor potential channel, PKD2L1, is reported to be a candidate receptor for sour taste based on molecular biological and functional studies. Here, we investigated the expression pattern of PKD2L1-immunoreactivity (IR) in taste buds of the mouse. PKD2L1-IR is present in a few elongate cells in each taste bud as reported previously. The PKD2L1-expressing cells are different from those expressing PLC beta 2, a marker of Type II cells. Likewise PKD2L1-immunoreactive taste cells do not express ecto-ATPase which marks Type I cells. The PKD2L1-positive cells are immunoreactive for neural cell adhesion molecule, serotonin, PGP-9.5 (ubiquitin carboxy-terminal transferase), and chromogranin A, all of which are present in Type III taste cells. At the ultrastructural level, PKD2L1-immunoreactive cells form synapses onto afferent nerve fibers, another feature of Type III taste cells. These results are consistent with the idea that different taste cells in each taste bud perform distinct functions. We suggest that Type III cells are necessary for transduction and/or transmission of information about "sour", but have little or no role in transmission of taste information of other taste qualities.