Changes of early post-traumatic osteoarthritis in an ovine model of simulated ACL reconstruction are associated with transient acute post-injury synovial inflammation and tissue catabolism
Changes of early post-traumatic osteoarthritis in an ovine model of simulated ACL reconstruction are associated with transient acute post-injury synovial inflammation and tissue catabolism
复制标题
DOI：
10.1016/j.joca.2013.08.019
复制
发表时间：
2013-12-01
影响因子：
7
通讯作者：
Frank, C. B.
中科院分区：
文献类型：
--
作者：
Heard, B. J.;Solbak, N. M.;Frank, C. B.
本文描述的研究在绵羊模型中检验了早期关节内炎症与创伤后骨关节炎（PTOA）的发展相关的假设。我们扩展了先前发表的工作，其中我们研究了解剖前交叉韧带（ACL）自体移植重建手术（ACL-R）后2周的关节大体形态和炎症和分解代谢分子的滑膜mRNA表达。在术后20周时分析了相同的变量以及两个时间点的新实验变量。在ACL-R手术后20周处死动物，并对它们的关节进行PTOA体征分级。收集滑膜样本进行组织学分级，并对一组炎症和分解代谢分子进行mRNA和蛋白质分析。还在软骨样品中研究了该组加上结缔组织基质转换分子的mRNA表达水平。术后20周的大体形态学评估结果显示与早期OA一致的一些变化，但表明从2周时间点开始几乎没有损伤进展。虽然在2周时检测到滑膜炎性和分解代谢分子的mRNA水平的显著变化，但到20周时数值已正常化。同样，在ACL-R手术后20周，关节软骨中炎性和分解代谢分子的所有mRNA表达水平都恢复到正常水平。我们的结论是，滑膜炎性过程开始后非常早的ACL-R手术，并可能煽动事件，导致严重的软骨和关节异常观察早在2周。然而，没有持续的炎症和关节不稳定可能会阻止OA进展。(C)2013年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
The study described here tested the hypothesis that early intra-articular inflammation is associated with the development of post-traumatic osteoarthritis (PTOA) in a sheep model. We extended previously published work in which we investigated joint gross morphology and synovial mRNA expression of inflammatory and catabolic molecules 2 weeks after anatomic Anterior cruciate ligament (ACL) autograft reconstructive surgery (ACL-R). The same variables have been analyzed at 20 weeks post surgery together with new experimental variables at both time points. Animals were sacrificed at 20 weeks post ACL-R surgery and their joints graded for signs of PTOA. Synovial samples were harvested for histological grading plus mRNA and protein analysis for a panel of inflammatory and catabolic molecules. The mRNA expression levels for this panel plus connective tissue matrix turnover molecules were also investigated in cartilage samples. Results of gross morphological assessments at 20 weeks post surgery showed some changes consistent with early OA, but indicated little progression of damage from the 2 week time point. While significant alterations in mRNA levels for synovial inflammatory and catabolic molecules were detected at 2 weeks, values had normalized by 20 weeks. Similarly, all mRNA expression levels for inflammatory and catabolic molecules in articular cartilage had returned to normal levels by 20 weeks post ACL-R surgery. We conclude that synovial inflammatory processes are initiated very early after ACL-R surgery and may instigate events that lead to the gross cartilage and joint abnormalities observed as early as 2 weeks. However, the absence of sustained inflammation and joint instability may prevent OA progression. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.